Individual project

Funder: German Research Foundation

Period: 2003-2007

URI: https://gepris.dfg.de/gepris/projekt/5410689

Abstract: 
mRNA-processing in bacteria and eukarya is a highly-ordered process which contributes to the regulation of gene expression. We have previously shown that as well salt concentration as growth phase influence the stability of some gvp-transcripts for gas vesicle synthesis but not of others in Haloferax. This implies that mRNA-processing contributes to regulated gene expression in archaea. We now want to perform a global analysis of mRNA-stabilities and their dependence of salt concentration and growth phase in Halobacterium salinarum by using microarrays. Furthermore we will apply three different approaches for the identification of mRNA-processing enzymes: 1) overexpression and characterization of H. salinarum proteins which are homologs to bacterial or eukaryal proteins with RNase or helicase function, 2) classical purification and characterization of RNA-processing activities from H. salinarum, 3) search for interaction partners of the identified proteins, since as well in bacteria as in eukarya large multi