Journalartikel

Jahr der Veröffentlichung: 1997

Seiten: 127-132

Zeitschrift: Journal of Plant Physiology

Bandnummer: 150

Heftnummer: 1-2

ISSN: 0176-1617

DOI Link: https://doi.org/10.1016/S0176-1617(97)80191-5

Verlag: Elsevier

Abstract: 
We have studied a possible function of jasmonates as mediators in the host-pathogen interaction of barley (Hordeum vulgare L.) with the powdery mildew fungus Egh (Erysiphe graminis f. sp. hordei). Previous findings from whole-leaf extracts demonstrated that (i) extracts from infected barley leaves did not contain enhanced levels of jasmonates, (ii) transcripts of jasmonate-inducible genes were not expressed upon infection, and (iii) exogenous application of jasmonates did not induce resistance to Egh (Kogel et al., 1995). Nevertheless, the question arises whether or not jasmonates are involved in the interaction of barley with the powdery mildew fungus at the local site of infection. Using an immunocytological approach the analysis of leaf cross-sections from a susceptible barley cultivar and its near-isogenic mlo(5)-resistant line revealed no accumulation of JIP-23, the most abundant jasmonate inducible protein, neither in epidermal cells attacked by the pathogen nor in adjacent mesophyll cells. As a positive control, cross-sections from methyl jasmonate-treated leaf segments showed a strong signal for JIP-23 accumulation. Because the presence of the jasmonate-inducible protein is highly indicative for an already low threshold level of endogenous jasmonate (Lehmann et al., 1995), the lack of JIP-23 accumulation at the sites of attempted fungal infection clearly demonstrates the absence of enhanced levels of jasmonates. This excludes even a local rise of jasmonate confined to those single cells penetrated (Mlo genotype) or attacked (mlo(5) genotype) by the fungus.

Harvard-Zitierstil: Hause, B., Kogel, K., Parthier, B. and Wasternack, C. (1997) In barley leaf cells, jasmonates do not act as a signal during compatible or incompatible interactions with the powdery mildew fungus (Erysiphe graminis f. sp. hordei), Journal of Plant Physiology, 150(1-2), pp. 127-132. https://doi.org/10.1016/S0176-1617(97)80191-5

APA-Zitierstil: Hause, B., Kogel, K., Parthier, B., & Wasternack, C. (1997). In barley leaf cells, jasmonates do not act as a signal during compatible or incompatible interactions with the powdery mildew fungus (Erysiphe graminis f. sp. hordei). Journal of Plant Physiology. 150(1-2), 127-132. https://doi.org/10.1016/S0176-1617(97)80191-5