A β-1,3-D-endo-mannanase from culture filtrates of the hyperparasites Verticillium lecanii and Aphanocladium album that specifically lyses the germ pore plug from uredospores of Puccinia graminis f.sp. tritici - Research portal of Justus Liebig University Giessen:

Journal article

A β-1,3-D-endo-mannanase from culture filtrates of the hyperparasites Verticillium lecanii and Aphanocladium album that specifically lyses the germ pore plug from uredospores of Puccinia graminis f.sp. tritici

Authors list: Langen, G; Beibmann, B; Reisener, HJ; Kogel, KH

Publication year: 1992

Pages: 853-860

Journal: Canadian Journal of Botany

Volume number: 70

Issue number: 4

ISSN: 0008-4026

DOI Link: https://doi.org/10.1139/b92-109

Publisher: National Research Council Canada

Abstract:
The hyperparasites Verticillium lecanii (Zimm.) Viegas syn. Verticillium hemileiae and Aphanocladium album (Preub) W. Gams are capable of penetrating the hyphal walls and uredospores of the stem rust fungus Puccinia graminis f.sp. tritici, their potential host. The culture filtrates of both hyperparasites contain an array of polysaccharide-degrading enzymes that in concert, digest the walls of uredospores completely. We have now isolated an enzyme with beta-1,3-D-endo-mannanase activity that is secreted by both hyperparasites into the culture medium in the presence of autoclaved walls of uredospores as a carbon source. The beta-mannanase predominately lyses the germ pore plugs of uredospores as shown by electron scanning microscopy. The enzyme has an apparent molecular mass of 19 000 Da as revealed by medium pressure liquid chromatography using a Superose 12 gel filtration column. An enzyme with identical specificity was found in dormant and soaked uredospores. Since opening of the germ pore plug is a very early and crucial step in germination of uredospores, the implication of beta-mannanases in the germination process is discussed.

Authors/Editors

- Prof. Dr. Karl-Heinz Kogel

Citation Styles

Harvard Citation style: Langen, G., Beibmann, B., Reisener, H. and Kogel, K. (1992) A β-1,3-D-endo-mannanase from culture filtrates of the hyperparasites Verticillium lecanii and Aphanocladium album that specifically lyses the germ pore plug from uredospores of Puccinia graminis f.sp. tritici, Canadian Journal of Botany, 70(4), pp. 853-860. https://doi.org/10.1139/b92-109

APA Citation style: Langen, G., Beibmann, B., Reisener, H., & Kogel, K. (1992). A β-1,3-D-endo-mannanase from culture filtrates of the hyperparasites Verticillium lecanii and Aphanocladium album that specifically lyses the germ pore plug from uredospores of Puccinia graminis f.sp. tritici. Canadian Journal of Botany. 70(4), 853-860. https://doi.org/10.1139/b92-109