Journal article

Publication year: 1991

Pages: 164-169

Journal: Planta

Volume number: 183

Issue number: 2

ISSN: 0032-0935

URL: https://www.jstor.org/stable/23380716

Publisher: Springer

Abstract: 
We have recently reported the isolation and characterization of a glycoprotein (M(r) 67 000) from germ-tube walls of Puccinia graminis f. sp. tritici which elicits the cellular hypersensitive lignification response in wheat (G. Kogel et al., 1988, Physiol. Mol. Plant Pathol. 33, 173-185). The present study uses this glycoprotein, referred to as Pgt elicitor, to identify putative elicitor targets in wheat cell membranes. In enzyme-linked immunosorbent assays using anti-Pgt elicitor antibodies, specific binding sites for Pgt elicitor were detected in highly purified plasma-membrane vesicles of wheat (Triticum aestivum L.) primary leaf cells. Binding proved to be independent of the presence or absence in wheat of the Sr5 gene for rust resistance, and also occurred on barley (Hordeum vulgare L.) plasma membrane. The binding sites have an Mr of 30 000 and 33 000, respectively, and binding activity was not lost in the presence of sodium dodecyl sulfate. [C-14]imido-Pgt elicitor was used to determine the apparent K(d) value for specific binding, found to be 2.0-mu-M, and the maximum content of binding sites, found to be 250 pmol per mg of plasma-membrane protein. The relevance of the elicitor binding for the outcome of the interaction of P. graminis and wheat is discussed.

Harvard Citation style: Kogel, G., Beissmann, B., Reisener, H. and Kogel, K. (1991) Specific binding of a hypersensitive lignification elicitor from Puccinia graminis f. sp. tritici to the plasma membrane from wheat (Triticum aestivum L.), Planta, 183(2), pp. 164-169. https://www.jstor.org/stable/23380716

APA Citation style: Kogel, G., Beissmann, B., Reisener, H., & Kogel, K. (1991). Specific binding of a hypersensitive lignification elicitor from Puccinia graminis f. sp. tritici to the plasma membrane from wheat (Triticum aestivum L.). Planta. 183(2), 164-169. https://www.jstor.org/stable/23380716