Journalartikel

Jahr der Veröffentlichung: 1988

Seiten: 173-185

Zeitschrift: Physiological and Molecular Plant Pathology

Bandnummer: 33

Heftnummer: 2

ISSN: 0885-5765

DOI Link: https://doi.org/10.1016/0885-5765(88)90018-5

Verlag: Elsevier

Abstract: 
Con A-binding glycoproteins were detected in germ tube walls of Puccinia grammis f. sp. tritici and appeared to be potent inducers of the hypersensitive lignification response. This response, which is typical of the large resistance reaction in wheat leaves, was preceded by a increase in extractable phenylalanine ammonia-lyase (PAL) actiity, a key enzyme of the phenylpropanoid pathway. Germ tube glycoproteins were isolated from homogenized fungal cell walls by differential centrifugation and affinity chromatography on a Con A-Sepharose column. The most active glycoprotein was purified on a Mono Q-anion-exchange column. The relative molecular mass of the molecule was determined in SDS-polyacrylamide gels to be 67 kD. The carbohydrate portion consists mainly of mannose (50%) and galactose (47%) and contains the active part of the glycoprotein as demonstrated by the inability of pronase and trypsin digestion to influence activity. An elicitor-active glycoprotein with identical molecular mass and Con A-binding properties was isolated from intercellular fluids of rust-infected plants of the susceptible wheat cultivar Little Club indicating that the elicitor is released from fungal cell walls during infection. We discuss the role of Con A-binding proteins as inducers of non-host resistance in cereals.

Harvard-Zitierstil: Kogel, G., Beissmann, B., Reisener, H. and Kogel, K. (1988) A single glycoprotein from Puccinia graminis f. sp. tritici cell walls elicits the hypersensitive lignification response in wheat, Physiological and Molecular Plant Pathology, 33(2), pp. 173-185. https://doi.org/10.1016/0885-5765(88)90018-5

APA-Zitierstil: Kogel, G., Beissmann, B., Reisener, H., & Kogel, K. (1988). A single glycoprotein from Puccinia graminis f. sp. tritici cell walls elicits the hypersensitive lignification response in wheat. Physiological and Molecular Plant Pathology. 33(2), 173-185. https://doi.org/10.1016/0885-5765(88)90018-5