An Elicitor of the Hypersensitive Lignification Response in Wheat Leaves Isolated from the Rust Fungus Puccinia graminis f. sp. tritici - I. Partial Purification and Characterization - Research portal of Justus Liebig University Giessen:

Journal article

An Elicitor of the Hypersensitive Lignification Response in Wheat Leaves Isolated from the Rust Fungus Puccinia graminis f. sp. tritici - I. Partial Purification and Characterization

Authors list: Moerschbacher, B; Kogel, KH; Noll, U; Reisener, HJ

Publication year: 1986

Pages: 830-838

Journal: A Journal of Biosciences

Volume number: 41c

Issue number: 9-10

ISSN: 0939-5075

eISSN: 1865-7125

DOI Link: https://doi.org/10.1515/znc-1986-9-1006

Publisher: De Gruyter

Abstract:
Several biotic and abiotic elicitors of the hypersensitive reaction in wheat leaves are described. The elicitors induce enhanced activity of phenylalanine ammonia-lyase with subsequent lignification, visible as a yellow autofluorescence. The deposited material stains positively with phloroglucinol. DEAE-dextran, epoxystearic acid and chitosan are strong elicitors, while the glucans tested have no activity. A biotic elicitor (genuine Pgt-elictor) was isolated from the germ tube walls of uredospores of Puccinia graminis f. sp. tritici. The high molecular weight, water soluble elicitor is heat stable and unaffected by mild acid and mild alkaline treatments. It seems to be a glycoprotein with the carbohydrate moiety bearing the active residues, as indicated by periodate sensitivity and protease stability. The carbohydrate moiety consists mainly of glucose with some galactose and mannose. Upon ultrafiltration and gel chromatogrpahy elicitor activity was associated with fractions of molecular weight of more than 100 kD. When injected into the intercellular space of primary wheat leaves, the elicitor induces lignification, preceded by an increase in phenylalanine ammonia-lyase activity.

Authors/Editors

- Prof. Dr. Karl-Heinz Kogel

Citation Styles

Harvard Citation style: Moerschbacher, B., Kogel, K., Noll, U. and Reisener, H. (1986) An Elicitor of the Hypersensitive Lignification Response in Wheat Leaves Isolated from the Rust Fungus Puccinia graminis f. sp. tritici - I. Partial Purification and Characterization, Zeitschrift fur Naturforschung C, 41c(9-10), pp. 830-838. https://doi.org/10.1515/znc-1986-9-1006

APA Citation style: Moerschbacher, B., Kogel, K., Noll, U., & Reisener, H. (1986). An Elicitor of the Hypersensitive Lignification Response in Wheat Leaves Isolated from the Rust Fungus Puccinia graminis f. sp. tritici - I. Partial Purification and Characterization. Zeitschrift fur Naturforschung C. 41c(9-10), 830-838. https://doi.org/10.1515/znc-1986-9-1006