Journal article

Publication year: 2016

Pages: 3345-3354

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 66

Issue number: 9

ISSN: 1466-5026

eISSN: 1466-5034

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.001200

Publisher: Microbiology Society

Abstract: 
Four yellow-pigmented, Gram-stain-negative, rod-shaped bacteria, strains PP-WC-4G-234(T), PP-CE-2G-454(T), PP-WC-1G-202 and PP-CC-3G-650, were isolated from the phyllosphere of Galium album. The strains shared 99.7-100% 16S rRNA gene sequence similarity but could be differentiated by genomic fingerprinting using rep-and random amplification of polymorphic DNA PCRs. Phylogenetic analysis based on the 16S rRNA gene placed the strains within the family Aurantimonadaceae with highest 16S rRNA gene sequence similarity of 97.2-97.3% to the type strain of Aureimonas phyllosphaerae. Sequence similarities to all other Aurantimonadaceae were below 97 %. The main cellular fatty acids of the strains were C-18 : 1 omega 7c as the predominant fatty acid followed by C-16 : 0 and summed feature 3 (C-16 : 1 omega 7c/C-16 : 1 omega 8c). The polyamine patterns of strains PP-WC-4G-234(T) and PP-CE-2G-454(T) contained sym-homospermidine as a major compound, and the major respiratory quinone was ubiquinone Q-10. Predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine, sulfoquinovosyldiacylglycerol, three unidentified phospholipids and one unidentified lipid only detectable after total lipid staining. The DNA G+C content was 66.4, 68.9, 67.4 and 70.5 mol% for strains PP-WC-4G-234(T), PP-CE-2G-454(T), PP-WC-1G-202 and PP-CC-3G-650, respectively. Based on phylogenetic, chemotaxonomic and phenotypic analyses we propose two novel species of the genus Aureimonas, Aureimonas galii sp. nov. with PP-WC-4G-234(T) (= LMG 28655(T) = CIP 110892(T)) as the type strain and Aureimonas pseudogalii sp. nov. with PP-CE-2G-454(T) (= LMG 29411(T) = CCM 8665(T)) as the type strain and two further strains representing the same species, PP-WC-1G-202 and PP-CC-3G-650.

Harvard Citation style: Aydogan, E., Busse, H., Moser, G., Müller, C., Kämpfer, P. and Glaeser, S. (2016) Aureimonas galii sp. nov. and Aureimonas pseudogalii sp. nov. isolated from the phyllosphere of Galium album, International Journal of Systematic and Evolutionary Microbiology, 66(9), pp. 3345-3354. https://doi.org/10.1099/ijsem.0.001200

APA Citation style: Aydogan, E., Busse, H., Moser, G., Müller, C., Kämpfer, P., & Glaeser, S. (2016). Aureimonas galii sp. nov. and Aureimonas pseudogalii sp. nov. isolated from the phyllosphere of Galium album. International Journal of Systematic and Evolutionary Microbiology. 66(9), 3345-3354. https://doi.org/10.1099/ijsem.0.001200