Journal article

Publication year: 2015

Pages: 1633-1638

Journal: Antonie van Leeuwenhoek

Volume number: 107

Issue number: 6

ISSN: 0003-6072

DOI Link: https://doi.org/10.1007/s10482-015-0434-2

Publisher: Springer

Abstract: 
An intense yellow pigmented strain (SUR2(T)) isolated from dehydrated activated sludge was studied in detail to clarify its taxonomic assignment. Cells of the isolate showed a rod-shaped morphology and stained Gram-negative. Comparative 16S rRNA gene sequence analysis revealed highest similarities to the type strains of Chryseobacterium polytrichastri YG4-6(T) (98.6 %), Chryseobacterium aahli T68F(T) (97.9 %), Chryseobacterium daeguense K105(T) and Chryseobacterium gregarium DSM 79109(T) (both 97.4 %). 16S rRNA gene-sequence similarities to all other Chryseobacterium species were below 97.3 %. The fatty acid analysis of strain SUR2(T) revealed a Chryseobacterium typical profile composed mainly of the fatty acids C15:0 iso, C15:0 iso 2-OH, C17:1 iso ω9c, and C17:0 iso 3-OH. DNA-DNA hybridizations with the type strains of C. polytrichastri, C. aahli, C. daeguense and C. gregarium resulted in values below 70 %. Differentiating biochemical and chemotaxonomic properties showed differences to the most closely related species and suggest that the isolate SUR2(T) represents a novel species, for which the name Chryseobacterium limigenitum sp. nov. (type strain SUR2(T) = ZIM B1019(T) = CCM 8594(T) = LMG 28734(T)) is proposed.

Harvard Citation style: Kämpfer, P., Trček, J., Skok, B., Šorgo, A. and Glaeser, S. (2015) Chryseobacterium limigenitum sp. nov., isolated from dehydrated sludge., Antonie van Leeuwenhoek, 107(6), pp. 1633-1638. https://doi.org/10.1007/s10482-015-0434-2

APA Citation style: Kämpfer, P., Trček, J., Skok, B., Šorgo, A., & Glaeser, S. (2015). Chryseobacterium limigenitum sp. nov., isolated from dehydrated sludge.. Antonie van Leeuwenhoek. 107(6), 1633-1638. https://doi.org/10.1007/s10482-015-0434-2