Journal article

Publication year: 1999

Pages: 261-267

Journal: Journal of Industrial Microbiology and Biotechnology

Volume number: 23

Issue number: 4-5

ISSN: 1367-5435

Open access status: Bronze

DOI Link: https://doi.org/10.1038/sj.jim.2900768

Publisher: Oxford University Press

Abstract: 
The increasing significance of members of the genus Sphingomonas in biotechnological applications has led to an increased interest in the diversity, abundance and ecophysiological potential of this group of Gram-negative bacteria. This general focus provides a challenge to improve means for identification of sphingomonads; eg molecular genetic methods for rapid and specific detection could facilitate screening of new isolates. Here, fluorescently labeled oligonucleotide probes targeted against 16S rRNA were used to typify strains previously assigned to the genus. All 46 sphingomonads tested including type strains of 21 Sphingomonasspecies could be detected with a probe originally designed for the genus and all but one with a probe designed for the alpha-4 subgroup of the Proteobacteria. The two probes are suitable for direct detection of sphingomonads in pure and mixed cultures as well as in environmental samples of unknown composition. The probes were used to identify sphingomonads in situ in activated sludge samples. Sphingomonads were rather abundant accounting for about 5-10% of the total cells in municipal sludges. Distinct patterns in aggregation of the cells suggest that these organisms could be involved in the formation process of sludge flocs.

Harvard Citation style: Neef, A., Witzenberger, R. and Kämpfer, P. (1999) Detection of sphingomonads and in situ identification in activated sludge using 16S rRNA-targeted oligonucleotide probes, Journal of Industrial Microbiology and Biotechnology, 23(4-5), pp. 261-267. https://doi.org/10.1038/sj.jim.2900768

APA Citation style: Neef, A., Witzenberger, R., & Kämpfer, P. (1999). Detection of sphingomonads and in situ identification in activated sludge using 16S rRNA-targeted oligonucleotide probes. Journal of Industrial Microbiology and Biotechnology. 23(4-5), 261-267. https://doi.org/10.1038/sj.jim.2900768