Journal article

Publication year: 2011

Pages: 2670-2675

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 61

Issue number: 11

ISSN: 1466-5026

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijs.0.026393-0

Publisher: Microbiology Society

Abstract: 
The taxonomic position, growth characteristics and antibiotic resistance properties of a slightly yellow-pigmented bacterial strain, designated R26(T), isolated from the midgut of the mosquito Anopheles gambiae, were studied. The isolate produced rod-shaped cells, which stained Gram-negative. The bacterium had two growth optima at 30-31 °C and 37 °C. Strain R26(T) demonstrated natural antibiotic resistance to ampicillin, chloramphenicol, kanamycin, streptomycin and tetracycline. 16S rRNA gene sequence analysis revealed that the isolate showed 98.6 % sequence similarity to that of Elizabethkingia meningoseptica ATCC 13253(T) and 98.2 % similarity to that of Elizabethkingia miricola GTC 862(T). The major fatty acids of strain R26(T) were iso-C(15 : 0), iso-C(17 : 0) 3-OH and summed feature 4 (iso-C(15 : 0) 2-OH and/or C(16 : 1)ω7c/t). Strain R26(T) contained only menaquinone MK-6 and showed a complex polar lipid profile consisting of diphosphatidylglycerol, phosphatidylinositol, an unknown phospholipid and unknown polar lipids and glycolipids. DNA-DNA hybridization experiments with E. meningoseptica CCUG 214(T) ( = ATCC 13253(T)) and E. miricola KCTC 12492(T) ( = GTC 862(T)) gave relatedness values of 34.5 % (reciprocal 41.5 %) and 35.0 % (reciprocal 25.7 %), respectively. DNA-DNA hybridization results and some differentiating biochemical properties indicate that strain R26(T) represents a novel species, for which the name Elizabethkingia anophelis sp. nov. is proposed. The type strain is R26(T) ( = CCUG 60038(T) = CCM 7804(T)).

Harvard Citation style: Kämpfer, P., Matthews, H., Glaeser, S., Martin, K., Lodders, N. and Faye, I. (2011) Elizabethkingia anophelis sp. nov., isolated from the midgut of the mosquito Anopheles gambiae., International Journal of Systematic and Evolutionary Microbiology, 61(11), pp. 2670-2675. https://doi.org/10.1099/ijs.0.026393-0

APA Citation style: Kämpfer, P., Matthews, H., Glaeser, S., Martin, K., Lodders, N., & Faye, I. (2011). Elizabethkingia anophelis sp. nov., isolated from the midgut of the mosquito Anopheles gambiae.. International Journal of Systematic and Evolutionary Microbiology. 61(11), 2670-2675. https://doi.org/10.1099/ijs.0.026393-0