Journal article

Publication year: 2002

Pages: 1655-1661

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 52

Issue number: 5

ISSN: 1466-5026

eISSN: 1466-5034

DOI Link: https://doi.org/10.1099/00207713-52-5-1655

Publisher: Microbiology Society

Abstract: 
Two facultatively oligotrophic, intensely yellow-pigmented bacterial strains, RE35F/1T and RE10F/45, have been previously isolated from the western Mediterranean Sea (Bay of Calvi, Corsica, France) by 0.2 microm membrane filtration. The organisms were gram-negative, catalase- and oxidase-positive, strictly aerobic, rod-shaped and non-motile. Their respiratory lipoquinone profiles consisted exclusively of ubiquinone-10 (Q-10) and the G+C contents of their DNAs were 62.0 and 62.4 mol%, respectively. Among the cellular fatty acids, octadecenoic acid (18:1omega7c) was the major component. Both isolates also contained hydroxy fatty acids (14:0 2-OH, 18:1 2-OH and 16:0 iso 3-OH) and branched fatty acids (15:0 anteiso, 16:0 anteiso and 17:0 anteiso). Polar lipid fingerprints were characterized by the presence of a sphingoglycolipid. Comparative analyses of their 16S rRNA gene sequences indicated that both isolates were phylogenetically closely related (sequence similarity of 99.9%) and formed a coherent cluster with aerobic bacteriochlorophyll a-containing species of the Erythrobacter/Porphyrobacter/Erythromicrobium cluster within the family Sphingomonadaceae. The closest relative was Erythrobacter litoralis DSM 8509T (97.4 and 97.5% 16S rRNA gene sequence similarity between this strain and RE35F/1T and RE10F/45, respectively). DNA-DNA reassociation studies confirmed that strains RE35F/1T and RE10F/45 represent a single species (79.6% DNA homology), but also demonstrated that they do not belong to the species Erythrobacter litoralis (25.2 and 34.2% DNA homology, respectively). Notably, both RE35F/1T and RE10F/45 lacked bacteriochlorophyll a. Based upon phenotypic and molecular evidence, a novel species of the genus Erythrobacter, Erythrobacter citreus sp. nov., is proposed. Strain RE35F/1T (= CIP 107092T = DSM 14432T) is the type strain.

Harvard Citation style: Denner, E., Vybiral, D., Koblízek, M., Kämpfer, P., Busse, H. and Velimirov, B. (2002) Erythrobacter citreus sp. nov., a yellow-pigmented bacterium that lacks bacteriochlorophyll a, isolated from the western Mediterranean Sea., International Journal of Systematic and Evolutionary Microbiology, 52(5), pp. 1655-1661. https://doi.org/10.1099/00207713-52-5-1655

APA Citation style: Denner, E., Vybiral, D., Koblízek, M., Kämpfer, P., Busse, H., & Velimirov, B. (2002). Erythrobacter citreus sp. nov., a yellow-pigmented bacterium that lacks bacteriochlorophyll a, isolated from the western Mediterranean Sea.. International Journal of Systematic and Evolutionary Microbiology. 52(5), 1655-1661. https://doi.org/10.1099/00207713-52-5-1655