Journal article

Publication year: 2002

Pages: 875-881

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 52

Issue number: 3

ISSN: 1466-5026

eISSN: 1466-5034

DOI Link: https://doi.org/10.1099/00207713-52-3-875

Publisher: Microbiology Society

Abstract: 
Two bacterial strains, designated 1A-C(T) and 3A-1, were studied and, using these results and previously published data, taxonomically classified. Cells of the strains exhibited a rod-coccus cycle. The peptidoglycan determined for 1A-C(T) was of type A4alpha with lysine as the diagnostic cell-wall diamino acid and an interpeptide bridge of L-Lys <-- L-Glu. The menaquinone systems of the two strains contained MK-8(H4) (82-94%) and MK-7(H4) (3-11%). The polar lipid profiles consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, two unidentified phospholipids and an unidentified glycolipid. The fatty acid profiles contained predominantly ai-C15:0 and significant amounts of i-C14:0 and i-C15:1 fatty acids. Genomic fingerprints clearly distinguished strains 1A-C(T) and 3A-1 from each other. DNA-DNA relatedness between the two strains (92%) demonstrated that they are members of a single species. Analyses of the 16S rDNA sequences of strains IA-C(T) and 3A-1, which were almost identical (99.6% sequence similarity), and comparison with corresponding sequences demonstrated that they represent a novel lineage within the suborder Micrococcineae, most closely related to species of the genera Beutenbergia, Bogoriella and Cellulomonas (94.7-95.7% sequence similarity). The results demonstrate that the two strains are members of a single new genus and a single novel species. Thus, the name Georgenia muralis gen. nov., sp. nov. is proposed. The type strain is strain 1A-C(T) (= DSM 14418T = CCM 4963T). Another strain of the species is strain 3A-1 (= DSM 14419 = CCM 4964).

Harvard Citation style: Altenburger, P., Kämpfer, P., Schumann, P., Vybiral, D., Lubitz, W. and Busse, H. (2002) Georgenia muralis gen. nov., sp. nov., a novel actinobacterium isolated from a medieval wall painting., International Journal of Systematic and Evolutionary Microbiology, 52(3), pp. 875-881. https://doi.org/10.1099/00207713-52-3-875

APA Citation style: Altenburger, P., Kämpfer, P., Schumann, P., Vybiral, D., Lubitz, W., & Busse, H. (2002). Georgenia muralis gen. nov., sp. nov., a novel actinobacterium isolated from a medieval wall painting.. International Journal of Systematic and Evolutionary Microbiology. 52(3), 875-881. https://doi.org/10.1099/00207713-52-3-875