Journal article

Publication year: 2011

Pages: 237-241

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 61

Issue number: 2

ISSN: 1466-5026

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijs.0.021675-0

Publisher: Microbiology Society

Abstract: 
Two Gram-stain-positive, non-motile, non-spore-forming cocci (strains MK-7(T) and MPA-33(T)) were isolated from poultry houses. Strain MK-7(T) was isolated on marine broth agar from coquina, a food supplement for female ducks used in a duck-fattening farm. Strain MPA-33(T) was isolated from the air of a turkey house on TSA after filter sampling. On the basis of 16S rRNA gene sequence similarity studies, both strains were shown to belong to the genus Jeotgalicoccus; MK-7(T) was most closely related to Jeotgalicoccus psychrophilus YKJ-115(T) (99.3 % similarity) and MPA-33(T) was most closely related to Jeotgalicoccus halotolerans YKJ-101(T) (98.8 %). The quinone system of MK-7(T) was composed of equal amounts of menaquinones MK-7 and MK-6 and that of MPA-33(T) contained 76 % MK-7 and 24 % MK-6. The polar lipid profile of strain MK-7(T) consisted of the major compounds diphosphatidylglycerol and phosphatidylglycerol and six unidentified lipids present in minor to moderate amounts. In strain MPA-33(T), diphosphatidylglycerol was the single predominant lipid, whereas phosphatidylglycerol was detected in moderate amounts. In addition, one unidentified phospholipid and four unidentified lipids were detected. Fatty acid profiles with iso-15 : 0 and anteiso-15 : 0 as major fatty acids supported the affiliation of the strains to the genus Jeotgalicoccus. The results of physiological and biochemical tests as well as DNA-DNA hybridizations allowed clear phenotypic differentiation of strains MK-7(T) and MPA-33(T) from the most closely related species. Strains MK-7(T) and MPA-33(T) therefore represent novel species, for which the names Jeotgalicoccus coquinae sp. nov. (type strain MK-7(T) =DSM 22419(T) =CCM 7682(T) =CCUG 57956(T)) and Jeotgalicoccus aerolatus sp. nov. (type strain MPA-33(T) =DSM 22420(T) =CCM 7679(T) =CCUG 57953(T)) are proposed.

Harvard Citation style: Martin, E., Klug, K., Frischmann, A., Busse, H., Kämpfer, P. and Jäckel, U. (2011) Jeotgalicoccus coquinae sp. nov. and Jeotgalicoccus aerolatus sp. nov., isolated from poultry houses., International Journal of Systematic and Evolutionary Microbiology, 61(2), pp. 237-241. https://doi.org/10.1099/ijs.0.021675-0

APA Citation style: Martin, E., Klug, K., Frischmann, A., Busse, H., Kämpfer, P., & Jäckel, U. (2011). Jeotgalicoccus coquinae sp. nov. and Jeotgalicoccus aerolatus sp. nov., isolated from poultry houses.. International Journal of Systematic and Evolutionary Microbiology. 61(2), 237-241. https://doi.org/10.1099/ijs.0.021675-0