Journalartikel

Jahr der Veröffentlichung: 2005

Seiten: 583-588

Zeitschrift: International Journal of Systematic and Evolutionary Microbiology

Bandnummer: 55

Heftnummer: 2

ISSN: 1466-5026

eISSN: 1466-5034

DOI Link: https://doi.org/10.1099/ijs.0.63386-0

Verlag: Microbiology Society

Abstract: 
A polychlorophenol-degrading strain, designated MT1(T), and three MT1-like strains, MT101, MT103 and MT104, were isolated from a cold (4-8 degrees C) fluidized-bed process treating chlorophenol-contaminated groundwater in southern Finland. The organisms were Gram-negative, rod-shaped, catalase-positive, non-spore-forming and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strains belonged to the alpha-4 subclass of the Proteobacteria and were members of the genus Novosphingobium. The highest 16S rRNA gene sequence similarity observed for these strains was 96.5 % with the type strains of Novosphingobium hassiacum, Novosphingobium aromaticivorans and Novosphingobium subterraneum. Chemotaxonomic data (major ubiquinone: Q-10; major polyamine: spermidine; major polar lipids: phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sphingoglycolipid; major fatty acids: 18 : 1omega7c, 16 : 1omega7c and 2-OH 14 : 0) as well as the ability to reduce nitrate supported the affiliation of the strains to the genus Novosphingobium. Based on the phylogenetic analysis, whole-cell fatty acid composition as well as biochemical and physiological characteristics, the MT1-like strains were highly similar and could be separated from all recognized Novosphingobium species. The novel species Novosphingobium lentum sp. nov. is proposed to accommodate strains MT1(T) (=DSM 13663(T)=CCUG 45847(T)), MT101 (=CCUG 45849), MT103 (=CCUG 45850) and MT104 (=CCUG 45851).

Harvard-Zitierstil: Tiirola, M., Busse, H., Kämpfer, P. and Männistö, M. (2005) Novosphingobium lentum sp. nov., a psychrotolerant bacterium from a polychlorophenol bioremediation process, International Journal of Systematic and Evolutionary Microbiology, 55(2), pp. 583-588. https://doi.org/10.1099/ijs.0.63386-0

APA-Zitierstil: Tiirola, M., Busse, H., Kämpfer, P., & Männistö, M. (2005). Novosphingobium lentum sp. nov., a psychrotolerant bacterium from a polychlorophenol bioremediation process. International Journal of Systematic and Evolutionary Microbiology. 55(2), 583-588. https://doi.org/10.1099/ijs.0.63386-0