Journal article

Publication year: 2017

Pages: 1046-1051

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 67

Issue number: 4

ISSN: 1466-5026

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.001761

Publisher: Microbiology Society

Abstract: 
A lightly yellowish-pigmented, oxidase-negative bacterial strain (PB45.5T) isolated from the Nam Nao district of Phetchabun in central Thailand was investigated to determine its taxonomic position. Cells of the isolate showed a rod shaped appearance. The strain stained Gram-negative. Strain PB45.5T shared highest 16S rRNA gene sequence similarity with the type strains of Photorhabdus luminescens subsp. akhurstii (99.2 %) and Photorhabdus luminescens subsp. hainanensis (99.1 %) and lower similarities to all other Photorhabdus luminescens subspecies (<98.0 %). Multilocus sequence analysis (MLSA) based on concatenated partial recA, dnaN, gltX, gyrB and infB gene sequences confirmed the affiliation obtained by 16S rRNA gene sequence analysis but showed a clear distinction of PB45.5T from the closest related type strains. Strain PB45.5T shared only 96.9 % sequence similarity (concatenated nucleotide sequences) with P. luminescens subsp. akhurstii FRG04T and 96.8 % with P. luminescens subsp. hainanensis C8404T. The fatty acid profile of the strain consisted of the major fatty acids C14 : 0, C16 : 0, C17 : 0 cyclo, C16 : 1ω7c and/or iso-C15 : 0 2-OH, and C18 : 1ω7c. The MLSA results and the differential biochemical and chemotaxonomic properties showed that strain PB45.5T represents a novel P. luminescens subspecies, for which the name Photorhabdus luminescens subsp. namnaonensis subsp. nov. (type strain PB45.5T=LMG 29915T=CCM 8729T) is proposed.

Harvard Citation style: Glaeser, S., Tobias, N., Thanwisai, A., Chantratita, N., Bode, H. and Kämpfer, P. (2017) Photorhabdus luminescens subsp namnaonensis subsp nov., isolated from Heterorhabditis baujardi nematodes, International Journal of Systematic and Evolutionary Microbiology, 67(4), pp. 1046-1051. https://doi.org/10.1099/ijsem.0.001761

APA Citation style: Glaeser, S., Tobias, N., Thanwisai, A., Chantratita, N., Bode, H., & Kämpfer, P. (2017). Photorhabdus luminescens subsp namnaonensis subsp nov., isolated from Heterorhabditis baujardi nematodes. International Journal of Systematic and Evolutionary Microbiology. 67(4), 1046-1051. https://doi.org/10.1099/ijsem.0.001761