Journal article

Publication year: 2016

Pages: 2643-2649

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 66

Issue number: 7

ISSN: 1466-5026

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.001100

Publisher: Microbiology Society

Abstract: 
A yellow pigmented strain (JM-1081T) isolated from healthy stem tissue of Zea mays was taxonomically characterized. Cells of the strain appeared rod-shaped and stained Gram-negative. Comparative 16S rRNA gene sequence analysis revealed closest relationship to the type strains of Sphingobacterium multivorum (98.1%), Sphingobacerium mucilaginosum (97.9%), and Sphingobacterium siyangense (97.8%). The 16S rRNA gene sequence similarities to all other Sphingobacterium species were below 97.8%. Fatty acid analysis of whole cell hydrolysates of the strain resulted in a Sphingobacterium typical pattern with fatty acids iso-C15:0 2-OH and/or C16:1ω7, C15:0 iso, C17:0 iso 3-OH and C16:0 and as major compounds. The polyamine pattern contains predominantly sym-homospermidine. The major quinone is menaquinone MK-7 and the only identified lipids in the polar lipid profile are phosphatidylethanolamine and phosphatidylserine. In addition 15 unidentified lipids were detected in moderate to major amounts. Sphingolipid was detected. The diagnostic diamino acid of the peptidoglycan is meso-diaminopimelic acid. DNA-DNA hybridizations with two of the closely related type strains, S. multivorum and S. siyangense, as well as S. canadense resulted in values below 70%. In addition to the genotypic differences, differentiating biochemical and chemotaxonomic properties confirmed that the isolate JM-1081T represents a novel species, for which the name Sphingobacterium zeae sp. nov. (type strain JM-1081T = LMG 29191T = CCM 8652T) is proposed.

Harvard Citation style: Kämpfer, P., Busse, H., Kleinhagauer, T., McInroy, J. and Glaeser, S. (2016) Sphingobacterium zeae sp. nov. an endophyte of maize., International Journal of Systematic and Evolutionary Microbiology, 66(7), pp. 2643-2649. https://doi.org/10.1099/ijsem.0.001100

APA Citation style: Kämpfer, P., Busse, H., Kleinhagauer, T., McInroy, J., & Glaeser, S. (2016). Sphingobacterium zeae sp. nov. an endophyte of maize.. International Journal of Systematic and Evolutionary Microbiology. 66(7), 2643-2649. https://doi.org/10.1099/ijsem.0.001100