Journalartikel

Jahr der Veröffentlichung: 2017

Zeitschrift: International Journal of Systematic and Evolutionary Microbiology

Bandnummer: 67

Heftnummer: 5

ISSN: 1466-5034

Open Access Status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.001770

Verlag: Microbiology Society

Abstract: 
Two slightly yellowish pigmented, oxidase-negative, rod-shaped and Gram-negative bacterial strains (30TX1T and DL20T), isolated from Steinernema sangi and Steinernema eapokense, respectively, during soil sampling in Vietnam were studied in a polyphasic taxonomic approach. Strain 30TX1T showed highest 16S rRNA gene sequence similarity to Xenorhabdus ehlersii (98.9%) and strain DL20T to Xenorhabdus ishibashii (98.7%). Sequence similarities to all other Xenorhabdus species were lower (< 98.4%). The two strains shared 98% 16S rRNA gene sequence similarity. Multilocus sequence analyses (MLSA) based on concatenated partial recA, dnaN, gltX, gyrB and infB sequences showed a clear distinction of strains, 30TX1T and DL20T, among each other and to closest related type strains. DNA-DNA hybridizations between DL20T and type strain of X. ishibashii resulted in similarity values of 53%, respectively. Genome-to-genome based comparisons gave average nucleotide identities (ANI) of 93.6% (reciprocal 93.5%) for strain 30TX1T and X. ehlersii DSM 16337T, of 92.8% (reciprocal 93%) for strain DL20T and X. ishibashii and of 93.0% (reciprocal 93.2%) for the two strains. The fatty acid profile of the strains consisted of the major fatty acids C14:0, C16:0 ,C17:0 cyclo, C16:1 ω7c and/or iso-C15:0 2-OH and C18:1 ω7c. Genome-to-genome comparison and MLSA results and the differentiating biochemical and chemotaxonomic properties showed, that strains 30TX1T and DL20T, represent novel Xenorhabdus species, for which the names Xenorhabdus thuongxuanensis sp. nov. (type strain 30TX1T = CCM 8727T = LMG 29916T), and Xenorhabdus eapokensis sp. nov. (type strain DL20T = CCM 8728T = LMG 29917T) are proposed, respectively.

Harvard-Zitierstil: Kämpfer, P., Tobias, N., Ke, L., Bode, H. and Glaeser, S. (2017) Xenorhabdus thuongxuanensis sp. nov. and Xenorhabdus eapokensis sp. nov., isolated from Steinernema species., International Journal of Systematic and Evolutionary Microbiology, 67(5). https://doi.org/10.1099/ijsem.0.001770

APA-Zitierstil: Kämpfer, P., Tobias, N., Ke, L., Bode, H., & Glaeser, S. (2017). Xenorhabdus thuongxuanensis sp. nov. and Xenorhabdus eapokensis sp. nov., isolated from Steinernema species.. International Journal of Systematic and Evolutionary Microbiology. 67(5). https://doi.org/10.1099/ijsem.0.001770