Journal article

Publication year: 2014

Pages: 491-500

Journal: Antonie van Leeuwenhoek

Volume number: 105

Issue number: 3

ISSN: 0003-6072

eISSN: 1572-9699

DOI Link: https://doi.org/10.1007/s10482-013-0101-4

Publisher: Springer

Abstract: 
Four yellow pigmented strains (91A-561(T), 91A-576, 91A-593(T), and JM-1085(T)) isolated from plant materials, showed 97.2-98.7 % 16S rRNA gene sequence similarities among each other and were studied in a polyphasic approach for their taxonomic allocation. Cells of all four isolates were rod-shaped and stained Gram-negative. Comparative 16S rRNA gene sequence analysis showed that the four bacteria had highest sequence similarities to Chryseobacterium formosense (97.2-98.7 %), Chryseobacterium gwangjuense (97.1-97.8 %), and Chryseobacterium defluvii (94.6-98.0 %). Sequence similarities to all other Chryseobacterium species were below 97.5 %. Fatty acid analysis of the four strains showed Chryseobacterium typical profiles consisting of major fatty acids C-15:0 iso, C-15:0 iso 2-OH/C-16:1 omega 7c, C-17:1 iso omega 9c, and C-17:0 iso 3-OH, but showed also slight differences. DNA-DNA hybridizations with type strains of C. gwangjuense, C. formosense, and C. defluvii resulted in values below 70 %. Isolates 91A-561(T) and 91A-576 showed DNA-DNA hybridization values > 80 % indicating that they belonged to the same species; but nucleic acid fingerprinting showed that the two isolates represent two different strains. DNA-DNA hybridization results and the differentiating biochemical and chemotaxonomic properties showed, that both strains 91A-561(T) and 91A-576 represent a novel species, for which the name Chryseobacterium geocarposphaerae sp. nov. (type strain 91A-561(T)=LMG 27811(T)=CCM 8488(T)) is proposed. Strains 91A-593(T) and JM-1085(T) represent two additional new species for which we propose the names Chyrseobacterium zeae sp. nov. (type strain JM-1085(T)=LMG 27809(T), =CCM 8491(T)) and Chryseobacterium arachidis sp. nov. (type strain 91A-593(T)=LMG 27813(T), =CCM 8489(T)), respectively.

Harvard Citation style: Kämpfer, P., McInroy, J. and Glaeser, S. (2014) Chryseobacterium zeae sp. nov., Chryseobacterium arachidis sp. nov., and Chryseobacterium geocarposphaerae sp. nov. isolated from the rhizosphere environment., Antonie van Leeuwenhoek, 105(3), pp. 491-500. https://doi.org/10.1007/s10482-013-0101-4

APA Citation style: Kämpfer, P., McInroy, J., & Glaeser, S. (2014). Chryseobacterium zeae sp. nov., Chryseobacterium arachidis sp. nov., and Chryseobacterium geocarposphaerae sp. nov. isolated from the rhizosphere environment.. Antonie van Leeuwenhoek. 105(3), 491-500. https://doi.org/10.1007/s10482-013-0101-4