Journal article

Publication year: 2012

Pages: 215-225

Journal: Journal of The American Academy of Dermatology

Volume number: 67

Issue number: 2

ISSN: 0190-9622

DOI Link: https://doi.org/10.1016/j.jaad.2011.09.002

Publisher: Elsevier

Abstract: 

Background: Merkel cell carcinoma (MCC) is a rare, highly malignant neuroendocrine tumor of the skin characterized by frequent lymphatic metastasis. Objective We sought to identify lymphovascular anatomy and expression profiles of lymphangiogenic cytokines to give an opinion on lymphangiogenesis in MCC.
Methods: We studied lymphatic microanatomy and lymphangiogenic cytokines in 27 MCC by immunohistology or immunofluorescence (D2-40, lymphatic vessel endothelial hyaluronan receptor [LYVE-1], vascular endothelial growth factor [VEGF] receptor-3, VEGF-C, VEGF-D, Ki67/MiB-1, CD68/PG-M1, CD68/KP1, CD163), Merkel cell polyomavirus–specific polymerase chain reaction, and coanalysis with clinical and histologic data. Results: We found a more than 3-fold increase in the mean density of absolute numbers of small lymphatic capillaries (diameter <10 μm) and a more than 8-fold increase in the median ratio of the number of small to large lymphatics (<10/≥10 μm) paratumorally compared with intraindividual controls. VEGF-C+CD68+ CD163+ cells (interpreted as M2 macrophages) could be identified as an important potentially lymphangiogenesis-inducing cell type. Limitations Partially lacking follow-up data limited the analysis of the prognostic impact. Conclusions: Our findings strongly indicate lymphangiogenesis in MCC driven by VEGF-C+CD68+ CD163+ M2 macrophages.

Harvard Citation style: Werchau, S., Toberer, F., Enk, A., Dammann, R. and Helmbold, P. (2012) Merkel cell carcinoma induces lymphatic microvessel formation, Journal of The American Academy of Dermatology, 67(2), pp. 215-225. https://doi.org/10.1016/j.jaad.2011.09.002

APA Citation style: Werchau, S., Toberer, F., Enk, A., Dammann, R., & Helmbold, P. (2012). Merkel cell carcinoma induces lymphatic microvessel formation. Journal of The American Academy of Dermatology. 67(2), 215-225. https://doi.org/10.1016/j.jaad.2011.09.002