Journal article

Publication year: 2017

Pages: 4902-4910

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 67

Issue number: 12

ISSN: 1466-5026

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.002192

Publisher: Microbiology Society

Abstract: 
A yellow-pigmented, Gram-stain-negative, motile and rod-shaped bacterium, strain M1A16(T), was isolated from the internal tissue of a sponge of the genus Haliclona, which was long-term cultured in the CEMarin aquaria system at Justus Liebig University of Giessen. The strain grew well at 20-32 degrees C (optimum 25 degrees C), in the presence of 0-6% NaCl (optimum 3 %), and at pH 5.5-9.0 (optimum pH 7.0-8.0). Phylogenetic analysis based on its 16S rRNA gene sequence placed the strain within the monophyletic cluster of the genus Winogradskyella with highest sequence similarity to Winogradskyella jejuensis CP32(T) (98.3% 16S rRNA gene sequence similarity). Sequence similarities to all other type strains were 98.0% or less. DNA-DNA hybridization of strain M1A16(T) with W. jejuensis CP32(T) resulted in hybridization values of 44.1% (reciprocal 68.1 %). Major cellular fatty acids of strain M1A16(T) were iso-C-15 : 1 G (18.1 %), iso-C-15 : 0 (13.7 %), C-16 : 1 omega 7c (12.9 %), iso-C-17 : 0 3-OH (10.6 %) and iso-C-16 : 0 3-OH (10.2 %). The overall polyamine content was very low with major components being cadaverine, spermidine and sym-homospermidine. The major quinone was menaquinone MK-6. The polar lipid profile contained predominantly phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids devoid of a detectable functional group. The genomic DNA G+C content was 32.7 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic analyses, strain M1A16(T) represents a novel species of the genus Winogradskyella, for which the name Winogradskyella haliclonae sp. nov. is proposed. The type strain is M1A16(T) (=DSM 103138(T) =CCM 8681(T) =LMG 29588(T) =CIP 111091(T)).

Harvard Citation style: Schellenberg, J., Busse, H., Hardt, M., Schubert, P., Wilke, T., Kämpfer, P., et al. (2017) Winogradskyella haliclonae sp nov., isolated from a marine sponge of the genus Haliclona, International Journal of Systematic and Evolutionary Microbiology, 67(12), pp. 4902-4910. https://doi.org/10.1099/ijsem.0.002192

APA Citation style: Schellenberg, J., Busse, H., Hardt, M., Schubert, P., Wilke, T., Kämpfer, P., & Glaeser, S. (2017). Winogradskyella haliclonae sp nov., isolated from a marine sponge of the genus Haliclona. International Journal of Systematic and Evolutionary Microbiology. 67(12), 4902-4910. https://doi.org/10.1099/ijsem.0.002192