Journal article

Publication year: 1987

Pages: 3515-3520

Journal: The EMBO Journal

Volume number: 6

Issue number: 11

ISSN: 0261-4189

DOI Link: https://doi.org/10.1002/j.1460-2075.1987.tb02677.x

Publisher: EMBO Press

Abstract: 

It has been proposed that intercistronic stem and loop structures
located in the puf operon of the photosynthetic bacterium Rhodobacter
capsulatus account for segmental differences in transcript stability and
consequently, the differential expression of the B870 and reaction
center (RC) proteins encoded by puf. We report here that deletion of
these structures leads to a failure to detect as discrete fragments the
B870‐encoding 0.49 kb and 0.50 kb mRNA segments located upstream from
the site of the hairpins. The absence of these stable transcript
fragments is associated with altered stoichiometry of the B870 and RC
pigment‐protein complexes in the bacterial intracytoplasmic membrane and
a decreased rate of cell growth under photosynthetic conditions. These
results support the view that the hairpin loop structures of the puf
intercistronic region function in vivo to impede exoribonucleolytic
degradation of upstream mRNA and establish that segmental variations in
mRNA stability have a biologically important role in regulating the
expression of puf operon genes.

Harvard Citation style: Klug, G., Adams, C., Belasco, J., Doerge, B. and Cohen, S. (1987) Biological consequences of segmental alterations in mRNA stability: effects of deletion of the intercistronic hairpin loop region of the Rhodobacter capsulatus puf operon, The EMBO Journal, 6(11), pp. 3515-3520. https://doi.org/10.1002/j.1460-2075.1987.tb02677.x

APA Citation style: Klug, G., Adams, C., Belasco, J., Doerge, B., & Cohen, S. (1987). Biological consequences of segmental alterations in mRNA stability: effects of deletion of the intercistronic hairpin loop region of the Rhodobacter capsulatus puf operon. The EMBO Journal. 6(11), 3515-3520. https://doi.org/10.1002/j.1460-2075.1987.tb02677.x