Journalartikel

Jahr der Veröffentlichung: 1998

Seiten: 212-217

Zeitschrift: Molecular and General Genetics MGG

Bandnummer: 260

Heftnummer: 2-3

ISSN: 0026-8925

DOI Link: https://doi.org/10.1007/s004380050888

Verlag: Springer Verlag (Germany)

Abstract: 
By using an oligonucleotide mixture corresponding to a region highly conserved among alternative sigma factors we identified a new sigma factor gene (rpoH) from Rhodobacter capsulatus. This gene encodes a protein of 34 kDa with strong similarity to the RpoH (sigma(32)) factors from other bacterial species. It was not possible to inactivate the R. capsulatus rpoH gene by introducing a resistance cassette? implying that it is essential for growth. The 5' ends of the mRNAs were mapped to two sequences with similarity to an rpoH- and an rpoD-dependent promoter, respectively. The amounts of both these mRNAs increased after heat shock, but were unaffected by a decrease in oxygen tension. Western analysis using a sigma factor-specific antibody revealed the accumulation of a protein of about 34 kDa after heat shock? and an increase in the amounts of a protein with the same size after reduction of oxygen tension in R. capsulatus cultures.

Harvard-Zitierstil: Emetz, D. and Klug, G. (1998) Cloning and characterization of the rpoH gene of Rhodobacter capsulatus, Molecular and General Genetics MGG, 260(2-3), pp. 212-217. https://doi.org/10.1007/s004380050888

APA-Zitierstil: Emetz, D., & Klug, G. (1998). Cloning and characterization of the rpoH gene of Rhodobacter capsulatus. Molecular and General Genetics MGG. 260(2-3), 212-217. https://doi.org/10.1007/s004380050888