Journal article

Publication year: 1984

Pages: 319-325

Journal: Archives of Microbiology

Volume number: 139

Issue number: 4

ISSN: 0302-8933

DOI Link: https://doi.org/10.1007/BF00408373

Publisher: Springer

Abstract: 

A gene bank of the phototrophic bacterium Rhodopseudomonas capsulata was constructed using the binary plasmid system pRK290/pRK2013. Fragments of about 20 kb of chromosomal DNA of R. capsulata strain 37b4 were inserted into the cloning vector pRK290. The hybrid plasmids of the gene bank, maintained in Escherichia coli HB101 were transferred by conjugation to R. capsulata strains defective in the photosynthetic apparatus with frequencies of 5×10^-4 to 5×10^-2. Phototrophically growing transconjugants occurred with frequencies of 5×10^-7 to 5×10^-6. Recombination between the hybrid plasmids and the R. capsulata chromosome was shown. The hybrid plasmid pRCF1002, carrying a 25 kb insert of R. capsulata wild type DNA, was isolated from one E. coli clone of the gene bank. It reconstituted some bacteriochlorophyll- and photosynthetic negative mutants to phototrophic growth.

Harvard Citation style: Klug, G. and Drews, G. (1984) Construction of a gene bank of Rhodopseudomonas capsulata using a broad host range DNA cloning system, Archives of Microbiology, 139(4), pp. 319-325. https://doi.org/10.1007/BF00408373

APA Citation style: Klug, G., & Drews, G. (1984). Construction of a gene bank of Rhodopseudomonas capsulata using a broad host range DNA cloning system. Archives of Microbiology. 139(4), 319-325. https://doi.org/10.1007/BF00408373