Journalartikel

Jahr der Veröffentlichung: 1993

Seiten: 749-757

Zeitschrift: Molecular Microbiology

Bandnummer: 10

Heftnummer: 4

ISSN: 0950-382X

DOI Link: https://doi.org/10.1111/j.1365-2958.1993.tb00945.x

Verlag: Wiley

Abstract: 
The pigment-binding proteins of Rhodobacter capsulatus are encoded by the polycistronic puf and puc operons. Both operons show higher expression under low oxygen tension than under high oxygen tension in the wild-type strain. The Tn5 mutant strain AH2 shows only low levels of puf and puc mRNA under high and low oxygen tension, indicating that it lacks a gene product required for stimulation of puf and puc gene expression under low oxygen tension. The formation of wild-type levels of photosynthetic complexes and normal oxygen regulation could be restored by the expression in trans of a 1.7kb fragment of the R. capsulatus wild-type chromosome or by addition of 10 mug l-1 vitamin B12 to the growth medium. An open reading frame of 798 nucleotides containing the Tn5 insertion was identified on the 1.7kb fragment. This open reading frame shows no homology to known genes and has a remarkably high GC content of 76%.

Harvard-Zitierstil: Pollich, M., Jock, S. and Klug, G. (1993) Identification of a gene required for the oxygen‐regulated formation of the photosynthetic apparatus of Rhodobacter capsulatus, Molecular Microbiology, 10(4), pp. 749-757. https://doi.org/10.1111/j.1365-2958.1993.tb00945.x

APA-Zitierstil: Pollich, M., Jock, S., & Klug, G. (1993). Identification of a gene required for the oxygen‐regulated formation of the photosynthetic apparatus of Rhodobacter capsulatus. Molecular Microbiology. 10(4), 749-757. https://doi.org/10.1111/j.1365-2958.1993.tb00945.x