Journal article

Publication year: 2014

Pages: 855-864

Journal: RNA Biology

Volume number: 11

Issue number: 7

ISSN: 1547-6286

Open access status: Green

DOI Link: https://doi.org/10.4161/rna.29440

Publisher: Taylor and Francis Group

Abstract: 
All bacteria contain multiple exoribonucleases to ensure a fast breakdown of different RNA molecules, either for maturation or for complete degradation to the level of mononucleotides. This efficient RNA degradation plays pivotal roles in the post-transcriptional gene regulation, in RNA processing and maturation as well as in RNA quality control mechanisms and global adaption to stress conditions. Besides different 3-to-5 exoribonucleases mostly with overlapping functions in vivo many bacteria additionally possess the 5-to-3 exoribonuclease, RNase J, to date the only known bacterial ribonuclease with this activity. An RNA-seq approach was applied to identify specific targets of RNase J in the -proteobacterium Rhodobacter sphaeroides. Only few transcripts were strongly affected by the lack of RNase J implying that its function is mostly required for specific processing/degradation steps in this bacterium. The accumulation of diverse RNA fragments in the RNase J deletion mutant points to RNA features that apparently cannot be targeted by the conventional 3-exoribonucleases in Gram-negative bacteria.

Harvard Citation style: Rische-Grahl, T., Weber, L., Remes, B., Forstner, K. and Klug, G. (2014) RNase J is required for processing of a small number of RNAs in Rhodobacter sphaeroides, RNA Biology, 11(7), pp. 855-864. https://doi.org/10.4161/rna.29440

APA Citation style: Rische-Grahl, T., Weber, L., Remes, B., Forstner, K., & Klug, G. (2014). RNase J is required for processing of a small number of RNAs in Rhodobacter sphaeroides. RNA Biology. 11(7), 855-864. https://doi.org/10.4161/rna.29440