Journal article

Immunochemical identification of insect hemocyte populations : monoclonal antibodies distinguish four major hemocyte types in manduca sexta.


Authors listWillott, E; Trenczek, T; Thrower, LW; Kanost, MR

Publication year1994

Pages417-423

JournalEuropean Journal of Cell Biology

Volume number65

Issue number2

ISSN0171-9335

PublisherElsevier


Abstract
We have made 140 monoclonal antibodies to hemocytes (insect blood cells) from Manduca sexta. Four of these antibodies, when used in immunofluorescent microscopy of fixed hemocytes, distinguish the four main morphologically distinct hemocyte types. Plasmatocytes, granular cells, and oenocytoids are each recognized by a unique antibody specific to that type; spherulocytes are recognized by an antibody that also binds to plasmatocytes. When used in flow cytometry with nonfixed hemocytes, three of the four antibodies bind their respective cells; the oenocytoid marker failed to bind to any hemocytes. This set of four monoclonal antibodies may be useful for labeling individual cell types and for separating the different hemocyte types for further study of hemocyte functions.



Citation Styles

Harvard Citation styleWillott, E., Trenczek, T., Thrower, L. and Kanost, M. (1994) Immunochemical identification of insect hemocyte populations : monoclonal antibodies distinguish four major hemocyte types in manduca sexta., European Journal of Cell Biology, 65(2), pp. 417-423

APA Citation styleWillott, E., Trenczek, T., Thrower, L., & Kanost, M. (1994). Immunochemical identification of insect hemocyte populations : monoclonal antibodies distinguish four major hemocyte types in manduca sexta.. European Journal of Cell Biology. 65(2), 417-423.


Last updated on 2025-21-05 at 13:14