Journal article
Authors list: Willott, E; Trenczek, T; Thrower, LW; Kanost, MR
Publication year: 1994
Pages: 417-423
Journal: European Journal of Cell Biology
Volume number: 65
Issue number: 2
ISSN: 0171-9335
Publisher: Elsevier
Abstract:
We have made 140 monoclonal antibodies to hemocytes (insect blood cells) from Manduca sexta. Four of these antibodies, when used in immunofluorescent microscopy of fixed hemocytes, distinguish the four main morphologically distinct hemocyte types. Plasmatocytes, granular cells, and oenocytoids are each recognized by a unique antibody specific to that type; spherulocytes are recognized by an antibody that also binds to plasmatocytes. When used in flow cytometry with nonfixed hemocytes, three of the four antibodies bind their respective cells; the oenocytoid marker failed to bind to any hemocytes. This set of four monoclonal antibodies may be useful for labeling individual cell types and for separating the different hemocyte types for further study of hemocyte functions.
Citation Styles
Harvard Citation style: Willott, E., Trenczek, T., Thrower, L. and Kanost, M. (1994) Immunochemical identification of insect hemocyte populations : monoclonal antibodies distinguish four major hemocyte types in manduca sexta., European Journal of Cell Biology, 65(2), pp. 417-423
APA Citation style: Willott, E., Trenczek, T., Thrower, L., & Kanost, M. (1994). Immunochemical identification of insect hemocyte populations : monoclonal antibodies distinguish four major hemocyte types in manduca sexta.. European Journal of Cell Biology. 65(2), 417-423.