Journal article

Publication year: 2000

Pages: 401-405

Journal: Nature

Volume number: 407

Issue number: 6802

DOI Link: https://doi.org/10.1038/35030160

Publisher: Nature Research

Abstract: 

In metazoans, most pre-messenger RNAs contain introns that are removed by splicing. The spliced mRNAs are then exported to the cytoplasm. Recent studies showed that splicing promotes efficient mRNA export1, but the mechanism for coupling these two processes is not known. Here we show that Aly, the metazoan homologue of the yeast mRNA export factor Yra1p (ref. 2), is recruited to messenger ribonucleoprotein (mRNP) complexes generated by splicing. In contrast, Aly does not associate with mRNPs assembled on identical mRNAs that already have no introns or with heterogenous nuclear RNP (hnRNP) complexes. Aly is recruited during spliceosome assembly, and then becomes tightly associated with the spliced mRNP. Aly shuttles between the nucleus and cytoplasm, and excess recombinant Aly increases both the rate and efficiency of mRNA export in vivo. Consistent with its splicing-dependent recruitment, Aly co-localizes with splicing factors in the nucleus. We conclude that splicing is required for efficient mRNA export as a result of coupling between the splicing and the mRNA export machineries.

Harvard Citation style: Zhou, Z., Lou, M., Sträßer, K., Katahira, J., Hurt, E. and Reed, R. (2000) The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans, Nature, 407(6802), pp. 401-405. https://doi.org/10.1038/35030160

APA Citation style: Zhou, Z., Lou, M., Sträßer, K., Katahira, J., Hurt, E., & Reed, R. (2000). The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans. Nature. 407(6802), 401-405. https://doi.org/10.1038/35030160