Journal article

Publication year: 2017

Pages: 380-

Journal: Frontiers in Cellular Neuroscience

Volume number: 11

Open access status: Gold

DOI Link: https://doi.org/10.3389/fncel.2017.00380

Publisher: Frontiers Media

Abstract: 

Understanding the mechanisms involved in maintaining lifelong
neurogenesis has a clear biological and clinical interest. In the
present study, we performed olfactory nerve transection on larval Xenopus
to induce severe damage to the olfactory circuitry. We surveyed the
timing of the degeneration, subsequent rewiring and functional
regeneration of the olfactory system following injury. A range of
structural labeling techniques and functional calcium imaging were
performed on both tissue slices and whole brain preparations. Cell death
of olfactory receptor neurons and proliferation of stem cells in the
olfactory epithelium were immediately increased following lesion. New
olfactory receptor neurons repopulated the olfactory epithelium and once
again showed functional responses to natural odorants within 1 week
after transection. Reinnervation of the olfactory bulb (OB) by newly
formed olfactory receptor neuron axons also began at this time.
Additionally, we observed a temporary increase in cell death in the OB
and a subsequent loss in OB volume. Mitral/tufted cells, the second
order neurons of the olfactory system, largely survived, but transiently
lost dendritic tuft complexity. The first odorant-induced responses in
the OB were observed 3 weeks after nerve transection and the olfactory
network showed signs of major recovery, both structurally and
functionally, after 7 weeks.

Harvard Citation style: Hawkins, S., Weiss, L., Offner, T., Dittrich, K., Hassenklöver, T. and Manzini, I. (2017) Functional Reintegration of Sensory Neurons and Transitional Dendritic Reduction of Mitral/Tufted Cells during Injury-Induced Recovery of the Larval Xenopus Olfactory Circuit, Frontiers in Cellular Neuroscience, 11, p. 380. https://doi.org/10.3389/fncel.2017.00380

APA Citation style: Hawkins, S., Weiss, L., Offner, T., Dittrich, K., Hassenklöver, T., & Manzini, I. (2017). Functional Reintegration of Sensory Neurons and Transitional Dendritic Reduction of Mitral/Tufted Cells during Injury-Induced Recovery of the Larval Xenopus Olfactory Circuit. Frontiers in Cellular Neuroscience. 11, 380. https://doi.org/10.3389/fncel.2017.00380