Journal article

Publication year: 2014

Pages: 695-704

Journal: Analytical and Bioanalytical Chemistry

Volume number: 406

Issue number: 3

ISSN: 1618-2642

DOI Link: https://doi.org/10.1007/s00216-013-7496-7

Publisher: Springer

Abstract: 
Fungal secondary metabolites in both fruiting bodies and pellets from submerged cultures of basidiomycetes were analyzed by atmospheric pressure matrix-assisted laser desorption/ionization-mass spectrometry imaging at a lateral resolution of 15 mu m, a mass resolution of 140,000 at m/z 200 and a mass accuracy of better than 2 ppm. The striatals A, B, C, and D, and a number of erinacine type metabolites were detected in the basidiomycetes Cyathus striatus and Hericium erinaceus, respectively. The two fungi were selected as model species, as they are well-known for efficient production of terpenoid secondary metabolites with interesting biological activities, e.g., antibacterial, fungicidal, cytotoxic properties, and stimulating effects on nerve growth factor synthesis. The localization of metabolites revealed a mostly homogeneous distribution of the striatals in the pellets of C. striatus, while a concentration gradient, increasing to the center, was observed in the pellets of H. erinaceus. A mostly homogeneous distribution of metabolites was also found in the fruiting body of H. erinaceus.

Harvard Citation style: Bhandari, D., Shen, T., Römpp, A., Zorn, H. and Spengler, B. (2014) Analysis of cyathane-type diterpenoids from Cyathus striatus and Hericium erinaceus by high-resolution MALDI MS imaging, Analytical and Bioanalytical Chemistry, 406(3), pp. 695-704. https://doi.org/10.1007/s00216-013-7496-7

APA Citation style: Bhandari, D., Shen, T., Römpp, A., Zorn, H., & Spengler, B. (2014). Analysis of cyathane-type diterpenoids from Cyathus striatus and Hericium erinaceus by high-resolution MALDI MS imaging. Analytical and Bioanalytical Chemistry. 406(3), 695-704. https://doi.org/10.1007/s00216-013-7496-7