Journalartikel

Jahr der Veröffentlichung: 2000

Seiten: 145-153

Zeitschrift: Journal of Photochemistry and Photobiology B: Biology

Bandnummer: 56

Heftnummer: 2-3

ISSN: 1011-1344

DOI Link: https://doi.org/10.1016/S1011-1344(00)00067-1

Verlag: Elsevier

Abstract: 
The recombinant phytochrome of the moss Ceratodon purpureus (CP2) expressed in Saccharomyces cerevisiae and reconstituted with phycocyanobilin (PCB) was investigated using fluorescence spectroscopy. The pigment had an emission maximum at 670 nm at low temperature (85 K) and at 667 nm at room temperature (RT) and an excitation maximum at 650-652 nm at 85 K (excitation spectra could not be measured at RT). Both spectra had a half-band width of approx. 30-35 nm at 85 K. The fluorescence intensity revealed a steep temperature dependence with an activation energy of fluorescence decay (E-a) of 5.9-6.4 and 12.6-14.7 kJ mol(-1) in the interval from 85 to 210 K and from 210 to 275 K, respectively. The photochemical properties of CP2/PCB were characterised by the extent of the red-induced (lambda (a) = 639 nm) Pr conversion into the first photoproduct lumi-R at 85 K (gamma (1)) of approximate to0.07 and into Pfr at RT (gamma (2)) of approximate to0.7. From these characteristics, CP2/PCB can be attributed to the Pr " photochemical type with gamma (1) less than or equal to 0.05, which comprises the minor phyA fraction (phyA "), phyB, Adiantum phy1 and Synechocystis Cph1 in contrast to the major phyA' fraction (Pr' type with gamma (1) = 0.5). Within the Pr " type, it is closer to phyA " than to phyB and Cph1. (C) 2000 Elsevier Science S.A. All rights reserved.

Harvard-Zitierstil: Sineshchekov, V., Koppel, L., Hughes, J., Lamparter, T. and Zeidler, M. (2000) Recombinant phytochrome of the moss Ceratodon purpureus (CP2): fluorescence spectroscopy and photochemistry, Journal of Photochemistry and Photobiology B: Biology, 56(2-3), pp. 145-153. https://doi.org/10.1016/S1011-1344(00)00067-1

APA-Zitierstil: Sineshchekov, V., Koppel, L., Hughes, J., Lamparter, T., & Zeidler, M. (2000). Recombinant phytochrome of the moss Ceratodon purpureus (CP2): fluorescence spectroscopy and photochemistry. Journal of Photochemistry and Photobiology B: Biology. 56(2-3), 145-153. https://doi.org/10.1016/S1011-1344(00)00067-1