Journal article

Publication year: 1999

Pages: 641-650

Journal: Journal of Plant Physiology

Volume number: 154

Issue number: 5-6

ISSN: 0176-1617

DOI Link: https://doi.org/10.1016/S0176-1617(99)80239-9

Publisher: Elsevier

Abstract: 
Moss protonemal filaments provide a useful plant model system for physiological studies of single cells and, as gametophytes, are attractive targers for mutation analysis. With its ability to grow in darkness, the species Ceratodon purpureus has proven particularly useful in photobiology We describe an optimised transformation procedure for this species. The use of various selectable (HPT, NPT) and screenable (GUS, LUG, GFP) reporters was established and different expression vectors were constructed for both constitutive (P-Actin1) and tetracycline-regulated (P-Top10) gene expression. The fate of transgenes introduced into the cell was monitored utilising a GFP construct by observing the expression pattern throughout recovery from the transformation procedure and further development.

Harvard Citation style: Zeidler, M., Hartmann, E. and Hughes, J. (1999) Transgene expression in the moss Ceratodon purpureus, Journal of Plant Physiology, 154(5-6), pp. 641-650. https://doi.org/10.1016/S0176-1617(99)80239-9

APA Citation style: Zeidler, M., Hartmann, E., & Hughes, J. (1999). Transgene expression in the moss Ceratodon purpureus. Journal of Plant Physiology. 154(5-6), 641-650. https://doi.org/10.1016/S0176-1617(99)80239-9