Journal article

Publication year: 1994

Pages: 179-183

Journal: Photochemistry and Photobiology

Volume number: 60

Issue number: 2

ISSN: 0031-8655

DOI Link: https://doi.org/10.1111/j.1751-1097.1994.tb05088.x

Publisher: Wiley

Abstract: 
The photometer described here measures phytochrome photoreversibility of as many as 20 samples fully automatically. Monochromatic light of two laser diodes (670 and 780 nm) is used as a source for the measuring light beam. The whole measuring procedure is controlled by a computer linked to a lock-in amplifier. This arrangement permits a rather simple construction and allows automatic zeroing and sample change.In addition the application of a Triton phase partitioning method to allow phytochrome photoreversibility measurements of extracts of green tissue is described. A single phase partitioning with a chlorophyll/phytochrome mixture reduced chlorophyll concentration to less than 10%. Repeated phase partitioning of crude extracts from green moss protonemata reduced chlorophyll content to less than 2% while leaving the phytochrome concentration almost unchanged. In some instances this method has advantages over polyethyleneimine precipitation, which is commonly used to obtain chlorophyll-free extracts.

Harvard Citation style: Lamparter, T., Hughes, J. and Hartmann, E. (1994) A fully automated dual-wavelength photometer for phytochrome measurements and its application to phytochrome from chlorophyll-containing extracts, Photochemistry and Photobiology, 60(2), pp. 179-183. https://doi.org/10.1111/j.1751-1097.1994.tb05088.x

APA Citation style: Lamparter, T., Hughes, J., & Hartmann, E. (1994). A fully automated dual-wavelength photometer for phytochrome measurements and its application to phytochrome from chlorophyll-containing extracts. Photochemistry and Photobiology. 60(2), 179-183. https://doi.org/10.1111/j.1751-1097.1994.tb05088.x