Analysis of cyclic and acyclic analogs of retinol, retinoic acid, and retinal by laser desorption ionization-, matrix-assisted laser desorption ionization mass spectrometry, and UV/Vis spectroscopy - Forschungsportal der Justus-Liebig-Universität Gießen:

Journalartikel

Analysis of cyclic and acyclic analogs of retinol, retinoic acid, and retinal by laser desorption ionization-, matrix-assisted laser desorption ionization mass spectrometry, and UV/Vis spectroscopy

Autorenliste: Wingerath, T; Kirsch, D; Spengler, B; Stahl, W

Jahr der Veröffentlichung: 1999

Seiten: 232-242

Zeitschrift: Analytical Biochemistry

Bandnummer: 272

Heftnummer: 2

ISSN: 0003-2697

DOI Link: https://doi.org/10.1006/abio.1999.4194

Verlag: Elsevier

Abstract:
Laser desorption ionization (LDI)- and matrix-assisted laser desorption ionization (MALDI)-mass spectrometry (LDI-MS, MALDI-MS) at 337-nm laser wavelength were used to analyze retinol (vitamin A), retinoic acid, and retinal and their analogs 3-hydroxy-retinol, 3-hydroxyretinoic acid, 3-hydroxyretinal, 4-oxoretinol, 4-oxoretinoic acid, 4-oxoretinal, 3,4-didehydroretinol (vitamin A(2)), 3,4-didehydroretinoic acid, 3,4-didehydroretinal, acycloretinol, acycloretinoic acid, and acycloretinal. The compounds exhibit sufficient ionizability which allows to obtain mass spectra by LDI which are similar in quality to those obtained by MALDI. Mass spectra were recorded with a linear time-of-flight (TOF) instrument or a reflectron-type (RETOF) instrument in positive-ion mode. Under the conditions of LDI-MS the compounds form abundant radical molecular ions (M+.), whereas in the MALDI mass spectra abundant protonated molecular ions ([M + H](+)) are observed. Characteristic fragment ions provide additional structural information. High-performance liquid chromatography (HPLC) coupled with UV/Vis photodiode detection was used to assist in retinoid characterization. Synthesis of 3-hydroxyretinal, 4-oxoretinal, and acycloretinal was performed by oxidative cleavage of the all-trans-carotenoids of zeaxanthin, canthaxanthin, and lycopene.

Zitierstile

Harvard-Zitierstil: Wingerath, T., Kirsch, D., Spengler, B. and Stahl, W. (1999) Analysis of cyclic and acyclic analogs of retinol, retinoic acid, and retinal by laser desorption ionization-, matrix-assisted laser desorption ionization mass spectrometry, and UV/Vis spectroscopy, Analytical Biochemistry, 272(2), pp. 232-242. https://doi.org/10.1006/abio.1999.4194

APA-Zitierstil: Wingerath, T., Kirsch, D., Spengler, B., & Stahl, W. (1999). Analysis of cyclic and acyclic analogs of retinol, retinoic acid, and retinal by laser desorption ionization-, matrix-assisted laser desorption ionization mass spectrometry, and UV/Vis spectroscopy. Analytical Biochemistry. 272(2), 232-242. https://doi.org/10.1006/abio.1999.4194