Journal article

Publication year: 2013

Pages: 6959-6968

Journal: Analytical and Bioanalytical Chemistry

Volume number: 405

Issue number: 22

ISSN: 1618-2642

DOI Link: https://doi.org/10.1007/s00216-013-7180-y

Publisher: Springer

Abstract: 
An atmospheric pressure laser desorption/ionization mass spectrometry imaging ion source has been developed that combines high spatial resolution and high mass resolution for the in situ analysis of biological tissue. The system is based on an infrared laser system working at 2.94 to 3.10 mu m wavelength, employing a Nd:YAG laser-pumped optical parametrical oscillator. A Raman-shifted Nd:YAG laser system was also tested as an alternative irradiation source. A dedicated optical setup was used to focus the laser beam, coaxially with the ion optical axis and normal to the sample surface, to a spot size of 30 mu m in diameter. No additional matrix was needed for laser desorption/ionization. A cooling stage was developed to reduce evaporation of physiological cell water. Ions were formed under atmospheric pressure and transferred by an extended heated capillary into the atmospheric pressure inlet of an orbital trapping mass spectrometer. Various phospholipid compounds were detected, identified, and imaged at a pixel resolution of up to 25 mu m from mouse brain tissue sections. Mass accuracies of better than 2 ppm and a mass resolution of 30,000 at m/z = 400 were achieved for these measurements.

Harvard Citation style: Römpp, A., Schäfer, K., Guenther, S., Wang, Z., Köstler, M., Leisner, A., et al. (2013) High-resolution atmospheric pressure infrared laser desorption/ionization mass spectrometry imaging of biological tissue, Analytical and Bioanalytical Chemistry, 405(22), pp. 6959-6968. https://doi.org/10.1007/s00216-013-7180-y

APA Citation style: Römpp, A., Schäfer, K., Guenther, S., Wang, Z., Köstler, M., Leisner, A., Paschke, C., Schramm, T., & Spengler, B. (2013). High-resolution atmospheric pressure infrared laser desorption/ionization mass spectrometry imaging of biological tissue. Analytical and Bioanalytical Chemistry. 405(22), 6959-6968. https://doi.org/10.1007/s00216-013-7180-y