Journal article

Publication year: 1996

Pages: 517-528

Journal: Journal of Applied Bacteriology

Volume number: 80

Issue number: 5

DOI Link: https://doi.org/10.1111/j.1365-2672.1996.tb03251.x

Publisher: Blackwell

Abstract: 

The taxonomic and discriminatory power of RFLP analysis of PCR amplified
parts of rhizobial rrn operons was compared to those of genomic PCR
fingerprinting with arbitrary and repetitive primers. For this purpose,
the two methods were applied for characterization of a group of
bacterial isolates referred to as Rhizobium `hedysari'. As outgroups,
representatives of the family Rhizobiaceae, belonging to the Rhizobium
galegae, Rhizobium meliloti, Rhizobium leguminosarum and Agrobacterium
tumefaciens species were used. By the RFLP analysis of the PCR products
corresponding to the variable 5'-half of the 23S rRNA gene and of the
amplified spacer region between the 16S and 23S rRNA genes all Rh.
`hedysari' strains studied were tightly clustered together while the
outgroups were placed in an outer position. The PCR products of the 3'
end parts of the 23S rDNA did not show significant RFL polymorphism and
no species differentiation on their basis was possible. In parallel,
analysis of the same strains was performed by PCR amplification of their
total DNA with 19, 18 and 10 bp long arbitrary primers (AP-PCR) as well
as with single primers corresponding to several bacterial repetitive
sequences (rep-PCR). By both AP and rep-PCR an identification of every
particular strain was achieved. In general, all primers provided
taxonomic results that are in agreement with the species and group
assignments based on the RFLP analysis of the rrn operons. On the basis
of the results presented here it can be concluded that AP and rep-PCR
are more informative and discriminative than rDNA and RFLP analysis of
the rhizobial strains studied.

Harvard Citation style: Selenska-Pobell, S., Evguenieva-Hackenberg, E., Radeva, G., Squartini, A. and (1996) Characterization of Rhizobium `hedysari' by RFLP analysis of PCR amplified rDNA and by genomic PCR fingerprinting, Journal of Applied Bacteriology, 80(5), pp. 517-528. https://doi.org/10.1111/j.1365-2672.1996.tb03251.x

APA Citation style: Selenska-Pobell, S., Evguenieva-Hackenberg, E., Radeva, G., Squartini, A., & (1996). Characterization of Rhizobium `hedysari' by RFLP analysis of PCR amplified rDNA and by genomic PCR fingerprinting. Journal of Applied Bacteriology. 80(5), 517-528. https://doi.org/10.1111/j.1365-2672.1996.tb03251.x