Journal article

Publication year: 1995

Pages: 49-60

Journal: Journal of Applied Bacteriology

Volume number: 79

Issue number: 1

DOI Link: https://doi.org/10.1111/j.1365-2672.1995.tb03123.x

Publisher: Blackwell

Abstract: 

The genomes of five nitrogen‐fixing strains isolated from the vicinity of Bayreuth and named formerly Enterobacter agglomerans were studied and compared with the genomes of several Rahnella aquatilis strains as well as with one Pantoea agglomerans and one Ent. agglomerans
reference strains, obtained from different world collections; they all
were previously assumed to be related to this group of natural isolates.

By using the infrequently cutting restriction endonuclease XbaI, highly chracteristic fingerprints were obtained for each of the studied strains except two Ent. agglomerans
isolates which had identical fingerprints. By hybridization of the
resulting individual PFGE‐fingerprints with a rDNA probe, containing the
rrnB ribosomal RNA operon of Escherichia coli, the relationship
between the analysed strains was studied. It was shown that the natural
isolates are very closely related to the type strain of R. aquatilis—ATCC
33071. The genome sizes of all studied strains were estimated to be
between 4.4 and 5.8 Mb on the basis of the lengths of their XbaI
fragments. By a modification of the PFGE technique it was shown that the
analysed strains harbour one to three large and extra large plasmids
with sizes in the range 90 to 608 kb.

Harvard Citation style: Evguenieva-Hackenberg, E. and Selenska-Pobell, S. (1995) Genome analysis of five soil bacterial isolates named formerly Enterobacter agglomerans, Journal of Applied Bacteriology, 79(1), pp. 49-60. https://doi.org/10.1111/j.1365-2672.1995.tb03123.x

APA Citation style: Evguenieva-Hackenberg, E., & Selenska-Pobell, S. (1995). Genome analysis of five soil bacterial isolates named formerly Enterobacter agglomerans. Journal of Applied Bacteriology. 79(1), 49-60. https://doi.org/10.1111/j.1365-2672.1995.tb03123.x