Journalartikel

Jahr der Veröffentlichung: 1993

Seiten: 4395-4406

Zeitschrift: The Journal of Immunology

Bandnummer: 150

Heftnummer: 10

ISSN: 0022-1767

URL: http://www.jimmunol.org/content/150/10/4395

Verlag: American Association of Immunologists

Abstract: 
The suspension pro granulocyte/macrophage (SPGM1) cell line was established from a transplantable mouse progranulocytic/promacrophage tumor. Surprisingly, SPGM1 cells expressed a typical CD5 pre-B cell phenotype, being positive for Ly-1 (CD5), PB76, B220 (CD45RA), and the pre-B Ig receptor complex (muH chains, lambda5 and v(pre-B) surrogate L chains, and the IgMalpha (mb-1) and Igbeta (B29) co-receptor molecules). Southern Blot analysis revealed clonal rearrangement of the muH chain locus and germ-line L chain loci. SPGM1 formed blast cell-, macrophage-, and occasional granulocytic colonies in soft agar in the presence of murine IL-3. IL-3 also induced macrophage differentiation of SPGM1 cells in suspension cultures. The earliest changes were detectable at 24 h by Northern blot analysis. IL-3-treatment increased Mac1 mRNA, induced c-fms mRNA, and down-regulated mRNA for mu, lambda5, V(pre-B) and mb-1. After 2 to 4 days the cells were larger, strongly adherent, expressed the macrophage markers Mac1 and F4/80, had lost muH chain and PB76 surface expression, and readily phagocytosed latex beads. Thus SPGM1 has all the characteristic features of a CD5+ pre-B cell line. However, IL-3 predominantly induced SPGM1 to switch its differentiation program from a pre-B cell to a macrophage. This inducible, rapid switch of virtually the entire population provides a unique model for the molecular analysis of mechanisms involved in cell-fate determination.

Harvard-Zitierstil: Martin, M., Strasser, A., Baumgarth, N., Cicuttini, F., Welch, K., Salvaris, E., et al. (1993) A novel cellular model (SPGM 1) of switching between the pre-B cell and myelomonocytic lineages, The Journal of Immunology, 150(10), pp. 4395-4406. http://www.jimmunol.org/content/150/10/4395

APA-Zitierstil: Martin, M., Strasser, A., Baumgarth, N., Cicuttini, F., Welch, K., Salvaris, E., & Boyd, A. (1993). A novel cellular model (SPGM 1) of switching between the pre-B cell and myelomonocytic lineages. The Journal of Immunology. 150(10), 4395-4406. http://www.jimmunol.org/content/150/10/4395