Journalartikel

Jahr der Veröffentlichung: 2000

Seiten: 529-535

Zeitschrift: Planta

Bandnummer: 210

Heftnummer: 4

ISSN: 0032-0935

DOI Link: https://doi.org/10.1007/s004250050041

Verlag: Springer

Abstract: 
In protonemal tip cells of the moss Ceratodon pur purpureus (Hedw.) Brid., phototropism and chlorophyll accumulation are regulated by the photoreceptor phytochrome. The mutant ptr116 lacks both responses as a result of a defect in the biosynthesis of phytochromobilin, the chromophore of phytochrome, at the point of biliverdin formation. The rescue of the phototropic response and of chlorophyll synthesis were tested by injecting different substances into tip cells of ptr116. Microinjection was first optimised with the use of fluorescent dyes and an expression plasmid containing a green fluorescent protein (GFP) gene. Injected phycocyanobilin, which substitutes for phytochromobilin, rescued both the phototropic response and light-induced chlorophyll accumulation in ptr116. The same results were obtained when expression plasmids with heme oxygenase genes of rat (HO-I) and Arabidopsis thaliana (L.) Heynh. (HY1) were injected. Heme oxygenase catalyses the conversion of heme into biliverdin. Whereas HY1 has a plastid target sequence and is presumably transferred to plastids, HO-1 is proposed to be cytosolic. The data show that ptr116 lacks heme oxygenase enzyme activity and indicate that heme oxygenases of various origin are active in Ceratodon bilin synthesis. In addition, it can be inferred from the data that the intracellular localisation of the expressed heme oxygenase is not important since the plastid enzyme can be replaced by a cytosolic one.

Harvard-Zitierstil: Brücker, G., Zeidler, M., Kohchi, T., Hartmann, E. and Lamparter, T. (2000) Microinjection of heme oxygenase genes rescues phytochrome-chromophore-deficient mutants of the moss Ceratodon purpureus, Planta, 210(4), pp. 529-535. https://doi.org/10.1007/s004250050041

APA-Zitierstil: Brücker, G., Zeidler, M., Kohchi, T., Hartmann, E., & Lamparter, T. (2000). Microinjection of heme oxygenase genes rescues phytochrome-chromophore-deficient mutants of the moss Ceratodon purpureus. Planta. 210(4), 529-535. https://doi.org/10.1007/s004250050041