Differential detection of key enzymes of polyaromatic-hydrocarbon-degrading bacteria using PCR and gene probes. - Research portal of Justus Liebig University Giessen:

Journal article

Differential detection of key enzymes of polyaromatic-hydrocarbon-degrading bacteria using PCR and gene probes.

Authors list: Meyer, S; Moser, R; Neef, A; Stahl, U; Kämpfer, P

Publication year: 1999

Pages: 1731-1741

Journal: Microbiology

Volume number: 145

Issue number: 7

ISSN: 1350-0872

eISSN: 1465-2080

Open access status: Bronze

DOI Link: https://doi.org/10.1099/13500872-145-7-1731

Publisher: Microbiology Society

Abstract:
Bacteria with ability to degrade polyaromatic hydrocarbons (PAHs), isolated from wastewater and soil samples, were investigated for their taxonomic, physiological and genetic diversity. Eighteen isolates able to metabolize naphthalene or phenanthrene as sole carbon source were taxonomically affiliated to different subclasses of the Proteobacteria (Sphingomonas spp., Acidovorax spp., Comamonas spp. and Pseudomonas spp.) and to phyla of Gram-positive bacteria with low and high DNA G + C content (Paenibacillus sp. and Rhodococcus spp., respectively). Representatives of the genera Pseudomonas and Sphingomonas formed a remarkably high fraction of these isolates; 9 out of 18 strains belonged to these groups. Tests for enzyme activities showed that the majority of the isolates growing with PAHs as sole sources of carbon and energy had an active catechol 2,3-dioxygenase (C230). C230 specific activities were very diverse, ranging from 0.1 to 650 mU (mg protein)-1. Pseudomonas and Sphingomonas strains showed considerably higher activities than the other isolates. All PAH degraders were examined for the presence of an initial PAH dioxygenase and C230, which catalyse key steps of PAH degradation, by PCR amplification of gene fragments and subsequent hybridization. PCR primers and internal oligonucleotide probes were developed for the specific detection of the genes of Pseudomonas and Sphingomonas strains.

Authors/Editors

- Uni.-Prof. Dr. Dr. Peter Kämpfer

Citation Styles

Harvard Citation style: Meyer, S., Moser, R., Neef, A., Stahl, U. and Kämpfer, P. (1999) Differential detection of key enzymes of polyaromatic-hydrocarbon-degrading bacteria using PCR and gene probes., Microbiology, 145(7), pp. 1731-1741. https://doi.org/10.1099/13500872-145-7-1731

APA Citation style: Meyer, S., Moser, R., Neef, A., Stahl, U., & Kämpfer, P. (1999). Differential detection of key enzymes of polyaromatic-hydrocarbon-degrading bacteria using PCR and gene probes.. Microbiology. 145(7), 1731-1741. https://doi.org/10.1099/13500872-145-7-1731