Journal article

Publication year: 2011

Pages: 2278-2283

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 61

Issue number: 9

ISSN: 1466-5026

DOI Link: https://doi.org/10.1099/ijs.0.027631-0

Publisher: Microbiology Society

Abstract: 
Two Gram-negative, rod-shaped, non-spore-forming strains, designated 08RB2639(T) and 08RB2781-1, were isolated from a sheep (Ovis aries) and a domestic boar (Sus scrofa domestica), respectively. By 16S rRNA gene sequencing, the isolates revealed identical sequences and were shown to belong to the Alphaproteobacteria. They exhibited 97.8 % 16S rRNA gene sequence similarity with Ochrobactrum rhizosphaerae PR17(T), O. pituitosum CCUG 50899(T), O. tritici SCII24(T) and O. haematophilum CCUG 38531(T) and 97.4 % sequence similarity with O. cytisi ESC1(T), O. anthropi LMG 3331(T) and O. lupini LUP21(T). The recA gene sequences of the two isolates showed only minor differences (99.5 % recA sequence similarity), and strain 08RB2639(T) exhibited the highest recA sequence similarity with Ochrobactrum intermedium CCUG 24694(T) (91.3 %). The quinone system was ubiquinone Q-10, with minor amounts of Q-9 and Q-11, the major polyamines were spermidine, putrescine and sym-homospermidine and the major lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine, with moderate amounts of the Ochrobactrum-specific unidentified aminolipid AL2. The major fatty acids (>20 %) were C₁₈:₁ω7c and C₁₉:₀ cyclo ω8c. These traits were in excellent agreement with the assignment of the isolates to the genus Ochrobactrum. DNA-DNA relatedness and physiological and biochemical tests allowed genotypic and phenotypic differentiation from other members of the genus Ochrobactrum. Hence, it is concluded that the isolates represent a novel species, for which the name Ochrobactrum pecoris sp. nov. is proposed (type strain 08RB2639(T)  = DSM 23868(T)  = CCUG 60088(T)  = CCM 7822(T)).

Harvard Citation style: Kämpfer, P., Huber, B., Busse, H., Scholz, H., Tomaso, H., Hotzel, H., et al. (2011) Ochrobactrum pecoris sp. nov., isolated from farm animals., International Journal of Systematic and Evolutionary Microbiology, 61(9), pp. 2278-2283. https://doi.org/10.1099/ijs.0.027631-0

APA Citation style: Kämpfer, P., Huber, B., Busse, H., Scholz, H., Tomaso, H., Hotzel, H., & Melzer, F. (2011). Ochrobactrum pecoris sp. nov., isolated from farm animals.. International Journal of Systematic and Evolutionary Microbiology. 61(9), 2278-2283. https://doi.org/10.1099/ijs.0.027631-0