Journal article

Publication year: 2015

Journal: PLoS ONE

Volume number: 10

Issue number: 3

ISSN: 1932-6203

eISSN: 1932-6203

Open access status: Gold

DOI Link: https://doi.org/10.1371/journal.pone.0119791

Publisher: Public Library of Science

Abstract: 
The presence of extended-spectrum beta-lactamase (ESBL)-producing
Escherichia coli was investigated in input (manure from livestock
husbandry) and output samples of six German biogas plants in 2012 (one
sampling per biogas plant) and two German biogas plants investigated in
an annual cycle four times in 2013/2014. ESBL-producing Escherichia coli
were cultured by direct plating on CHROMagar ESBL from input samples in
the range of 100 to 104 colony forming units (CFU) per g dry weight but
not from output sample. This initially indicated a complete elimination
of ESBL-producing E. coli by the biogas plant process. Detected non
target bacteria were assigned to the genera Acinetobacter, Pseudomonas,
Bordetella, Achromobacter, Castellaniella, and Ochrobactrum. A selective
pre-enrichment procedure increased the detection efficiency of
ESBL-producing E. coli in input samples and enabled the detection in
five of eight analyzed output samples. In total 119 ESBL-producing E.
coli were isolated from input and 46 from output samples. Most of the E.
coli isolates carried CTX-M-type and/or TEM-type beta lactamases (94%),
few SHV-type beta lactamase (6%). Sixty-four blaCTX-M genes were
characterized more detailed and assigned mainly to CTX-M-groups 1 (85%)
and 9 (13%), and one to group 2. Phylogenetic grouping of 80 E. coli
isolates showed that most were assigned to group A (71%) and B1 (27%),
only one to group D (2%). Genomic fingerprinting and multilocus sequence
typing (MLST) showed a high clonal diversity with 41 BOX-types and 19
ST-types. The two most common ST-types were ST410 and ST1210.
Antimicrobial susceptibility testing of 46 selected ESBL-producing E.
coli revealed that several isolates were additionally resistant to other
veterinary relevant antibiotics and some grew on CHROMagar STEC but
shiga-like toxine (SLT) genes were not detected. Resistance to
carbapenems was not detected. In summary the study showed for the first
time the presence of ESBL-producing E. coli in output samples of German
biogas plants.

Harvard Citation style: Schauss, T., Glaeser, S., Gütschow, A., Dott, W. and Kämpfer, P. (2015) Improved detection of extended spectrum beta-lactamase (ESBL)-producing Escherichia coli in input and output samples of German biogas plants by a selective pre-enrichment procedure, PLoS ONE, 10(3), Article e011979. https://doi.org/10.1371/journal.pone.0119791

APA Citation style: Schauss, T., Glaeser, S., Gütschow, A., Dott, W., & Kämpfer, P. (2015). Improved detection of extended spectrum beta-lactamase (ESBL)-producing Escherichia coli in input and output samples of German biogas plants by a selective pre-enrichment procedure. PLoS ONE. 10(3), Article e011979. https://doi.org/10.1371/journal.pone.0119791