Sammelband

Herausgeberliste: Becker, A

Jahr der Veröffentlichung: 2013

ISBN: 978-1627032773

eISBN: 978-1-62703-278-0

DOI Link: https://doi.org/10.1007/978-1-62703-278-0

Serientitel: Methods in Molecular Biology

Serienzählung: 975

Abstract: 

Plants are amazing organisms to study, some are important sources for
pharmaceuticals, and others can help to elucidate molecular mechanisms
required for a plant’s development and its interactions with the biotic
or abiotic environment. Functional genomics is vastly lagging behind the
speed of genome sequencing as high-throughput gene function assays are
difficult to design, specifically for non-model plants. Bioinformatics
tools are useful for gene identification and annotation but are of
limited value for predictions concerning gene functions as gene
functions are uncovered best by experimental approaches.
Virus-Induced-Gene-Silencing (VIGS) is an easy to use, fast, and
reliable method to achieve down regulation of target gene expression. Virus-Induced Gene Silencing: Methods and Protocols provides
detailed protocols for VIGS experiments in several plant species
including model and non-model plants. Also included in this book are
recently developed protocols for VIGS-derived microRNA production in the
plant or protein over expression, as well as chapters devoted to
summarizing the molecular mechanisms of VIGS action and the vector
systems developed so far. Written in the successful Methods in Molecular Biology™
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible protocols, and notes on troubleshooting and
avoiding known pitfalls.

Harvard-Zitierstil: Becker, A. (ed.) (2013) Virus-Induced Gene Silencing : Methods and Protocols. Totowa: Humana Press. https://doi.org/10.1007/978-1-62703-278-0

APA-Zitierstil: Becker, A. (Ed.) (2013). Virus-Induced Gene Silencing : Methods and Protocols. Humana Press. https://doi.org/10.1007/978-1-62703-278-0