Journal article

Frequent occurrence of RASSF1A promoter hypermethylation and merkel cell polyomavirus in merkel cell carcinoma


Authors listHelmbold, P; Lahtz, C; Enk, A; Herrmann-Trost, P; Marsch, WCh; Kutzner, H; Dammann, RH

Publication year2009

Pages903-909

JournalMolecular Carcinogenesis

Volume number48

Issue number10

ISSN0899-1987

eISSN1098-2744

Open access statusHybrid

DOI Linkhttps://doi.org/10.1002/mc.20540

PublisherWiley


Abstract

Merkel cell carcinoma (MCC) is one of the most aggressive cancers of the skin. It has recently been reported that integration of a Merkel cell polyomavirus (MCPyV) in receptor tyrosine phosphates type G (PTPRG) gene occurs in MCC, and that viral infections are associated with epigenetic silencing of tumor suppressor genes (TSG) in cancer. To examine whether a correlation between TSG inactivation and viral infection can be found in MCC, we investigated the promoter hypermethylation of RASSF1A, TP73, PTPRG, FHIT, and CDKN2A and the presence of MCPyV and SV40 in 98 MCC by PCR. Hypermethylation of RASSF1A was frequently found in 42 of 83 (51%) of MCC. Methylation of CDKN2A was present in 9 of 41 (22%) of MCC. Hypermethylation of TP73 (0%), PTPRG (4%), and FHIT (0%) was infrequent in MCC. Interestingly, MCPyV was found in 90 of 98 (92%) MCC, however, no SV40 signal was detected. No correlation between TSG hypermethylation and viral infection was found. Our results show frequent hypermethylation of RASSF1A and the presence of MCPyV in primary MCC, and that these events may contribute to the pathogenesis of MCC.




Citation Styles

Harvard Citation styleHelmbold, P., Lahtz, C., Enk, A., Herrmann-Trost, P., Marsch, W., Kutzner, H., et al. (2009) Frequent occurrence of RASSF1A promoter hypermethylation and merkel cell polyomavirus in merkel cell carcinoma, Molecular Carcinogenesis, 48(10), pp. 903-909. https://doi.org/10.1002/mc.20540

APA Citation styleHelmbold, P., Lahtz, C., Enk, A., Herrmann-Trost, P., Marsch, W., Kutzner, H., & Dammann, R. (2009). Frequent occurrence of RASSF1A promoter hypermethylation and merkel cell polyomavirus in merkel cell carcinoma. Molecular Carcinogenesis. 48(10), 903-909. https://doi.org/10.1002/mc.20540


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