Journalartikel

Jahr der Veröffentlichung: 2006

Seiten: 633-642

Zeitschrift: Thyroid

Bandnummer: 16

Heftnummer: 7

ISSN: 1050-7256

eISSN: 1557-9077

DOI Link: https://doi.org/10.1089/thy.2006.16.633

Verlag: Mary Ann Liebert

Abstract: 

Objective: To understand the role of epigenetic inactivation of tumor-related genes in the pathogenesis of thyroid cancer, we investigated the methylation profile of distinct thyroid neoplasms.
Design: We analyzed the methylation pattern of 17 gene promoters in nine thyroid cancer cell lines and in 38 primary thyroid carcinomas (13 papillary thyroid carcinoma [PTC], 10 follicular thyroid carcinoma [FTC], 9 undifferentiated thyroid carcinoma [UTC], 6 medullary thyroid carcinoma [MTC]), 12 goiters, and 10 follicular adenomas (FA) by methylation-specific polymerase chain reaction (PCR). Epigenetic inactivation was validated by expression analysis.
Main outcome: Twelve of these genes (RASSF1A, p16(INK4A), TSHR, MGMT, DAPK, ER alpha, ER beta, RAR beta, PTEN, CD26, SLC5A8, and UCHL1) were frequently methylated in UTC (15%-86%) and thyroid cancer cell lines (25%-100%). In the more aggressive UTC, the mean methylation index (MI 0.44) was the highest compared to other thyroid alterations PTC (MI = 0.29, p = 0.123), FTC (MI = 0.15, p = 0.005), MTC (MI = 0.13; p = 0.017), FA (MI = 0.27; p = 0.075) and goiters (MI = 0.23; p = 0.024). Methylation of TSHR, MGMT, UCHL1, and p16 occurred preferentially in UTC and this inactivation was reverted by a demethylating agent.
Conclusions: Our results show that hypermethylation of several tumor-related gene promoters is a frequent event in UTC. The hypermethylation status may be reversed by DNA demethylating agents. Their clinical value remains to be investigated.

Harvard-Zitierstil: Schagdarsurengin, U., Gimm, O., Dralle, H., Hoang-Vu, C. and Dammann, R. (2006) CpG island methylation of tumor-related promoters occurs preferentially in undifferentiated carcinoma, Thyroid, 16(7), pp. 633-642. https://doi.org/10.1089/thy.2006.16.633

APA-Zitierstil: Schagdarsurengin, U., Gimm, O., Dralle, H., Hoang-Vu, C., & Dammann, R. (2006). CpG island methylation of tumor-related promoters occurs preferentially in undifferentiated carcinoma. Thyroid. 16(7), 633-642. https://doi.org/10.1089/thy.2006.16.633