Combined actions of multiple hairpin loop structures and sites of rate-limiting endonucleolytic cleavage determine differential degradation rates of individual segments within polycistronic puf operon mRNA - Research portal of Justus Liebig University Giessen:

Journal article

Combined actions of multiple hairpin loop structures and sites of rate-limiting endonucleolytic cleavage determine differential degradation rates of individual segments within polycistronic puf operon mRNA

Authors list: Klug, G; Cohen, SN

Publication year: 1990

Pages: 5140-5146

Journal: Journal of Bacteriology

Volume number: 172

Issue number: 9

ISSN: 0021-9193

DOI Link: https://doi.org/10.1128/jb.172.9.5140-5146.1990

Publisher: American Society for Microbiology

Abstract:

Differential expression of the genes within the puf operon of
Rhodobacter capsulatus is accomplished in part by differences in the
rate of degradation of different segments of the puf transcript. We
report here that decay of puf mRNA sequences specifying the
light-harvesting I (LHI) and reaction center (RC) photosynthetic
membrane peptides is initiated endoribonucleolytically within a discrete
1.4-kilobase segment of the RC-coding region. Deletion of this segment
increased the half-life of the RC-coding region from 8 to 20 min while
not affecting decay of LHI-coding sequences upstream from an
intercistronic hairpin loop structure shown previously to impede
3'-to-5' degradation. Prolongation of RC segment half-life was dependent
on the presence of other hairpin structures 3' to the RC region.
Inserting the endonuclease-sensitive sites into the LHI-coding segment
markedly accelerated its degradation. Our results suggest that
differential degradation of the RC- and LHI-coding segments of puf mRNA
is accomplished at least in part by the combined actions of RC
region-specific endonuclease(s), one or more exonucleases, and several
strategically located exonuclease-impeding hairpins.

Authors/Editors

- Prof. Dr. Gabriele Klug

Citation Styles

Harvard Citation style: Klug, G. and Cohen, S. (1990) Combined actions of multiple hairpin loop structures and sites of rate-limiting endonucleolytic cleavage determine differential degradation rates of individual segments within polycistronic puf operon mRNA, Journal of Bacteriology, 172(9), pp. 5140-5146. https://doi.org/10.1128/jb.172.9.5140-5146.1990

APA Citation style: Klug, G., & Cohen, S. (1990). Combined actions of multiple hairpin loop structures and sites of rate-limiting endonucleolytic cleavage determine differential degradation rates of individual segments within polycistronic puf operon mRNA. Journal of Bacteriology. 172(9), 5140-5146. https://doi.org/10.1128/jb.172.9.5140-5146.1990