Journalartikel

Jahr der Veröffentlichung: 2012

Seiten: 5901-5909

Zeitschrift: Nucleic Acids Research

Bandnummer: 40

Heftnummer: 13

ISSN: 0305-1048

Open Access Status: Gold

DOI Link: https://doi.org/10.1093/nar/gks243

Verlag: Oxford University Press

Abstract: 
The expression of photosynthesis genes in the facultatively photosynthetic bacterium Rhodobacter sphaeroides is controlled by the oxygen tension and by light quantity. Two photoreceptor proteins, AppA and CryB, have been identified in the past, which are involved in this regulation. AppA senses light by its N-terminal BLUF domain, its C-terminal part binds heme and is redox-responsive. Through its interaction to the transcriptional repressor PpsR the AppA photoreceptor controls expression of photosynthesis genes. The cryptochrome-like protein CryB was shown to affect regulation of photosynthesis genes, but the underlying signal chain remained unknown. Here we show that CryB interacts with the C-terminal domain of AppA and modulates the binding of AppA to the transcriptional repressor PpsR in a light-dependent manner. Consequently, binding of the transcription factor PpsR to its DNA target is affected by CryB. In agreement with this, all genes of the PpsR regulon showed altered expression levels in a CryB deletion strain after blue-light illumination. These results elucidate for the first time how a bacterial cryptochrome affects gene expression.

Harvard-Zitierstil: Metz, S., Haberzettl, K., Fruhwirth, S., Teich, K., Hasewinkel, C. and Klug, G. (2012) Interaction of two photoreceptors in the regulation of bacterial photosynthesis genes, Nucleic Acids Research, 40(13), pp. 5901-5909. https://doi.org/10.1093/nar/gks243

APA-Zitierstil: Metz, S., Haberzettl, K., Fruhwirth, S., Teich, K., Hasewinkel, C., & Klug, G. (2012). Interaction of two photoreceptors in the regulation of bacterial photosynthesis genes. Nucleic Acids Research. 40(13), 5901-5909. https://doi.org/10.1093/nar/gks243