Journal article

Publication year: 2007

Pages: 2460-2471

Journal: Journal of Proteome Research

Volume number: 6

Issue number: 7

ISSN: 1535-3893

DOI Link: https://doi.org/10.1021/pr060624p

Publisher: American Chemical Society

Abstract: 
Singlet oxygen (O-1(2)) is a stress factor and signal in the facultative phototrophic bacterium Rhodobacter sphaeroides. In vivo protein labeling with L-[S-35]-methionine and analysis by two-dimensional gel electrophoresis revealed that the synthesis of 61 proteins was changed in response to O-1(2). After O-1(2) treatment, protein synthesis patterns were distinct from those after H2O2 treatment but similar to those after high light exposure. This indicates regulatory mechanisms selective for different reactive oxygen species (ROS) and a response to light partly mediated by O-1(2). Analysis of mutant strains support that the response to O-1(2) is regulated mainly by rpoE (sigma(E)), but also a modulation of the sigma(E) dependent response by other factors and the existence of sigma(E) independent responses. The involvement of the RNA chaperon Hfq in the O-1(2) response implies a role of small regulatory RNAs.

Harvard Citation style: Glaeser, J., Zobawa, M., Lottspeich, F. and Klug, G. (2007) Protein synthesis patterns reveal a complex regulatory response to singlet oxygen in Rhodobacter, Journal of Proteome Research, 6(7), pp. 2460-2471. https://doi.org/10.1021/pr060624p

APA Citation style: Glaeser, J., Zobawa, M., Lottspeich, F., & Klug, G. (2007). Protein synthesis patterns reveal a complex regulatory response to singlet oxygen in Rhodobacter. Journal of Proteome Research. 6(7), 2460-2471. https://doi.org/10.1021/pr060624p