Journal article

Publication year: 1988

Pages: 299-312

Journal: Insect biochemistry

Volume number: 18

Issue number: 3

ISSN: 0020-1790

DOI Link: https://doi.org/10.1016/0020-1790(88)90095-9

Publisher: Scientechnica Publ.

Abstract: 

Fat body from injured or immunized pupae as well as from untreated pupae of Hyalophora cecropia synthesize and release in vitro
the immune proteins P4, attacins, lysozyme and cecropins. During an
incubation time of 4 days immune protein synthesis declines relative to
the other proteins, i.e. protein synthesis returns to a normal pupal
pattern. Treatment of fat body in culture with bacterial compounds (LPS,
lyophilized cells of Micrococcus lysodeikticus or heat killed Escherichia coli),
but also sterile filtered insect Ringer, causes a relative higher rate
of synthesis of the immune proteins. At the same time synthesis of some
large proteins (75–100 kD) is switched off. Similar results are obtained
by adding hemocytes from injured or immunized pupae, whereas hemocytes
from untreated pupae show no effect. The induction of protein synthesis
with particular emphasis on the immune proteins will be discussed.

Harvard Citation style: Trenczek, T. and Faye, I. (1988) Synthesis of immune proteins in primary cultures of fat body from Hyalophora cecropia, Insect biochemistry, 18(3), pp. 299-312. https://doi.org/10.1016/0020-1790(88)90095-9

APA Citation style: Trenczek, T., & Faye, I. (1988). Synthesis of immune proteins in primary cultures of fat body from Hyalophora cecropia. Insect biochemistry. 18(3), 299-312. https://doi.org/10.1016/0020-1790(88)90095-9