Contribution in an anthology

Appeared in: Small Non-Coding RNAs : Methods and Protocols

Editor list: Rederstorff, M

Publication year: 2016

Pages: 29-38

ISBN: 978-1-4939-2546-9

eISBN: 978-1-4939-4903-8

DOI Link: https://doi.org/10.1007/978-1-4939-2547-6_4

Title of series: Methods in Molecular Biology

Number in series: 1296

Abstract: 

We prepared total RNA from the Gram-positive soil bacterium Bacillus subtilis
by different RNA extraction procedures to compare their suitability for
Northern blot detection of tiny RNAs (~14-mers) or RNAs of intermediate
size (100–200 nt) in terms of signal quality, intensity, and
reproducibility. Our analysis included two hot phenol methods and two
TRIzol extraction procedures. We found that signal intensity/detection
sensitivity makes the key difference. Total RNAs prepared by the hot
phenol method comprise the length spectrum from tRNAs to large ribosomal
RNAs. Larger RNAs are less abundant in TRIzol preparations which
instead enrich for RNAs of tRNA size and smaller. Thus, hot phenol
methods are the choice for the detection of intermediate-sized and
longer RNAs, whereas TRIzol extraction procedures are more suited for
the detection of tiny RNAs.

Harvard Citation style: Damm, K., Bach, S., Müller, K., Klug, G., Burenina, O., Kubareva, E., et al. (2016) Impact of RNA Isolation Protocols on RNA Detection by Northern Blotting, in Rederstorff, M. (ed.) Small Non-Coding RNAs : Methods and Protocols. New York: Springer Ney York - Humana Press, pp. 29-38. https://doi.org/10.1007/978-1-4939-2547-6_4

APA Citation style: Damm, K., Bach, S., Müller, K., Klug, G., Burenina, O., Kubareva, E., Grünweller, A., & Hartmann, R. (2016). Impact of RNA Isolation Protocols on RNA Detection by Northern Blotting. In Rederstorff, M. (Ed.), Small Non-Coding RNAs : Methods and Protocols (pp. 29-38). Springer Ney York - Humana Press. https://doi.org/10.1007/978-1-4939-2547-6_4