Sammelbandbeitrag

Erschienen in: Small Non-Coding RNAs : Methods and Protocols

Herausgeberliste: Rederstorff, M

Jahr der Veröffentlichung: 2016

Seiten: 41-51

ISBN: 978-1-4939-2546-9

eISBN: 978-1-4939-4903-8

DOI Link: https://doi.org/10.1007/978-1-4939-2547-6_5

Serientitel: Methods in Molecular Biology

Serienzählung: 1296

Abstract: 

Successful detection of very small RNAs (tiny RNA, ~14 nt in length) by
Northern blotting is dependent on improved Northern blot protocols that
combine chemical crosslinking of RNA with
1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) to positively
charged membranes, the use of native polyacrylamide gels, and the
development of highly sensitive and specific probes modified with locked
nucleic acids (LNA). In this protocol, we show that Northern blot
detection of tiny RNAs with 5′-digoxigenin-labeled DNA/LNA mixmer probes
is a highly sensitive and specific method and, in our hands, more
sensitive than using a corresponding DNA/LNA mixmer probe with a 5′-³²P-end label.

Harvard-Zitierstil: Damm, K., Bach, S., Müller, K., Klug, G., Burenina, O., Kubareva, E., et al. (2016) Improved Northern Blot Detection of Small RNAs Using EDC Crosslinking and DNA/LNA Probes, in Rederstorff, M. (ed.) Small Non-Coding RNAs : Methods and Protocols. New York: Springer Ney York - Humana Press, pp. 41-51. https://doi.org/10.1007/978-1-4939-2547-6_5

APA-Zitierstil: Damm, K., Bach, S., Müller, K., Klug, G., Burenina, O., Kubareva, E., Grünweller, A., & Hartmann, R. (2016). Improved Northern Blot Detection of Small RNAs Using EDC Crosslinking and DNA/LNA Probes. In Rederstorff, M. (Ed.), Small Non-Coding RNAs : Methods and Protocols (pp. 41-51). Springer Ney York - Humana Press. https://doi.org/10.1007/978-1-4939-2547-6_5